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| 产品名称： | 6-23 (Clone 6) |
|---|---|
| 商品货号： | TS212811 |
| Organism： | Rattus norvegicus, rat |
| Tissue： | thyroid |
| Cell Type： | C cell |
| Product Format： | frozen |
| Culture Properties： | adherent |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease： | medullary thyroid carcinoma |
| Storage Conditions： | liquid nitrogen vapor phase |
| Genes Expressed： | neurotensin; calcitonin |
| Comments： | Secretion of neurotensin and calcitonin is stimulated by calcium, KCl, glucagon and catecholamines. |
| Complete Growth Medium： | Dulbeccos modified Eagles medium with 4.5 g/L glucose, 85%; horse serum, 15%
|
| Subculturing： | Remove medium, add fresh 0.25% trypsin, 0.02% EDTA solution for 1 minute, remove trypsin EDTA, add 0.3 mL of 0.25% trypsin and let the culture sit at 37°C for 6 minutes. Add fresh medium, aspirate and dispense into new flasks. Subculture every 10 to 12 days.
Subcultivation Ratio: A subcultivation ratio of 1:10 to 1:20 is recommended
Medium Renewal: 3 times per week |
| Cryopreservation： | Freeze medium: complete growth medium, 95%; DMSO, 5% |
| Name of Depositor： | R Gagel, FN Zeytinoglu |
| Deposited As： | Rattus sp. |
| References： | Zeytinoglu FN, et al. Establishment of a calcitonin-producing rat medullary thyroid carcinoma cell line. Morphological studies of the tumor and cells in culture. Endocrinology 107: 509-515, 1980. PubMed: 6248326 Zeytinoglu FN, et al. Characterization of neurotensin production by a line of rat medullary thyroid carcinoma cells. Proc. Natl. Acad. Sci. USA 77: 3741-3745, 1980. PubMed: 6932046 |