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微信小章| 产品名称： | 70Z/3 70Z/3.12 |
|---|---|
| 商品货号： | TS212817 |
| Organism： | Mus musculus, mouse |
| Cell Type： | pre-B lymphoblast; methylnitrosourea induce |
| Product Format： | frozen |
| Morphology： | lymphoblast |
| Culture Properties： | suspension |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Storage Conditions： | liquid nitrogen vapor phase |
| Comments： | The cells are negative for surface immunoglobulin and terminal deoxynucleotidyl transferase (sIg -, TdT -). Tested and found negative for ectromelia virus (mousepox). |
| Complete Growth Medium： | The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium:
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| Subculturing： | Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 1 to 2 x 105 viable cells/mL. Maintain cell density between 1 x 105 and 1 x 106 viable cells/mL. Medium Renewal: Add fresh medium every 2 to 3 days (depending on cell density) |
| Cryopreservation： | Culture medium 95%; DMSO, 5%.xa0Cell culture tested DMSO is available as ATCC Catalog No. 4-X. |
| Culture Conditions： | Temperature: 37°C Atmosphere:xa05% CO2 |
| Name of Depositor： | PW Kincade |
| Deposited As： | Mus musculus |
| References： | Ralph P. T and B lymphocytes. New York: Academic Press; 1979. Baines P, et al. Characterization of malignant cell populations in MNU-induced leukaemia of mice. Leuk. Res. 3: 23-28, 1979. PubMed: 316844 Paige CJ, et al. Murine B cell leukemia line with inducible surface immunoglobulin expression. J. Immunol. 121: 641-647, 1978. PubMed: 308074 Paige CJ, et al. Independent control of immunoglobulin heavy and light chain expression in a murine pre-B-cell line. Nature 292: 631-633, 1981. PubMed: 6789213 Hay, R. J., Caputo, J. L., and Macy, M. L., Eds. (1992), ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC. Caputo, J. L., Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988. Fleming, D.O., Richardson, J. H., Tulis, J.J. and Vesley, D., (1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC. Biosafety in Microbiological and Biomedical Laboratories, 5th ed. HHS. U.S. Department of Health and Human Services, Centers for Disease Control and Prevention. Washington DC: U.S. Government Printing Office; 2007. The entire text is available online. |