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微信小章| 产品名称： | 5C6 Clone 1 |
|---|---|
| 商品货号： | TS212819 |
| Organism： | Rattus norvegicus (B cell); Rattus norvegicus (myeloma), rat (B cell); rat (myeloma) |
| Cell Type： | hybridoma: B lymphocyte |
| Product Format： | frozen |
| Morphology： | lymphoblast |
| Culture Properties： | suspension |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Applications： | The antibody reacts with the mouse myelomonocytic adhesion promoting complement receptor (CR3, CD11b). |
| Storage Conditions： | liquid nitrogen vapor phase |
| Derivation： | Spleen cells were fused with Y3 myeloma cells. |
| Clinical Data： | It inhibits myelomonocytic adhesion in vitro, and recruitment of inflammatory cells in vivo. |
| Genes Expressed： | immunoglobulin; monoclonal antibody; against mouse CD11b (type 3 complement receptor, CR3) |
| Cellular Products： | immunoglobulin; monoclonal antibody; against mouse CD11b (type 3 complement receptor, CR3) |
| Comments： |
mAb 5C6 was the product of a fusion between spleen cells from an AO rat, immunized four times with TPM, and fused with the Y3 rat myeloma line. The antibody reacts with the mouse myelomonocytic adhesion promoting complement receptor (CR3, CD11b).
It inhibits myelomonocytic adhesion in vitro, and recruitment of inflammatory cells in vivo. |
| Complete Growth Medium： | Iscoves modified Dulbeccos medium, 90%; fetal bovine serum, 10%
|
| Subculturing： | Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 x 105 cells/mL and maintain between 1 x 105 and 1 x 106 cells/mL.
Medium Renewal: Every 2 to 3 days |
| Cryopreservation： | 50% FBS + 40% complete growth medium + 10% DMSO. Cell culture tested DMSO is available as ATCC Catalog No. 4-X. |
| Culture Conditions： | Temperature: 37°C
Atmosphere: Air, 95%; Carbon dioxide (CO2), 5% |
| Isotype： | IgG2b |
| Name of Depositor： | H Rosen, S Gordon |
| Deposited As： | rat (B cell); rat (myeloma) |
| References： | Rosen H. Role of CR3 in induced myelomonocytic recruitment: insights from in vivo monoclonal antibody studies in the mouse. J. Leukocyte Biol. 48: 465-469, 1990. PubMed: 2230596 Hutchings P, et al. Transfer of diabetes in mice prevented by blockade of adhesion- promoting receptor on macrophages. Nature 348: 639-642, 1990. PubMed: 2250718 Rosen H, Gordon S. Monoclonal antibody to the murine type 3 complement receptor inhibits adhesion of myelomonocytic cells in vitro and inflammatory cell recruitment in vivo. J. Exp. Med. 166: 1685-1701, 1987. PubMed: 2445894 Hay, R. J., Caputo, J. L., and Macy, M. L., Eds. (1992), ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC. Caputo, J. L., Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988. Fleming, D.O., Richardson, J. H., Tulis, J.J. and Vesley, D., (1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC. |