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微信小章| 产品名称： | 5c8 |
|---|---|
| 商品货号： | TS212823 |
| Organism： | Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma) |
| Cell Type： | hybridoma: B lymphocyte |
| Product Format： | frozen |
| Morphology： | lymphoblast |
| Culture Properties： | suspension |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Strain： | Strain:xa0 BALB/c (B cell); BALB/c (myeloma) |
| Storage Conditions： | liquid nitrogen vapor phase |
| Disclosure： | This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC. |
| Derivation： | Spleen cells were fused with Sp2/0 myeloma cells. |
| Genes Expressed： | immunoglobulin; monoclonal antibody; against T-B activating molecule T-BAM, ligand for CD40 (CD40L, CD154) |
| Cellular Products： | immunoglobulin; monoclonal antibody; against T-B activating molecule T-BAM, ligand for CD40 (CD40L, CD154) |
| Comments： | Animals were immunized with D1.1 cells (see ATCC CRL-10915). Spleen cells were fused with Sp2/0 myeloma cells. The antibody recognizes an antigen present on T cells that is important to the T cell mediated activation of B cells. The antigen is a ligand for the CD40 molecule on the B cell surface. |
| Complete Growth Medium： | The base medium for this cell line is ATCC-formulated Dulbeccos Modified Eagles Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
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| Subculturing： | Protocol: Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10(5) cells/ml. Interval: Maintain between 1 X 10(5) and 1 X 10(6) cells/ml. Medium Renewal: Every 2 to 3 days |
| Cryopreservation： | Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO Storage temperature: liquid nitrogen vapor phase |
| Culture Conditions： | Atmosphere: air, 95%; carbon dioxide (CO2), 5% Temperature: 37.0°C |
| Isotype： | mouse IgG2a |
| Name of Depositor： | Trustees of Columbia University |
| Deposited As： | mouse (B cell); mouse (myeloma) |
| U.S. Patent Number： | |
| References： | Lederman S, et al. Murine monoclonal antibody (5c8) recognizes a human glycoprotein on the surface of T-lymphocytes, compositions containing same. US Patent 5,474,771 dated Dec 12 1995 Lederman S, et al. Methods for treating autoimmune diseases with 5C8-specific antibodies. US Patent 6,592,868 dated Jul 15 2003 . Protein recognized by an antibody that specifically binds an epitope that is specifically bound by monoclonal antibody 5c8. U.S. Patent 6,793,924 dated Sep 21 2004 |