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| 产品名称： | 5E4 |
|---|---|
| 商品货号： | TS212824 |
| Organism： | Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma) |
| Cell Type： | hybridoma: B lymphocyte |
| Product Format： | frozen |
| Morphology： | lymphoblast |
| Culture Properties： | suspension |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Applications： | VacA is a secreted protein that induces vacuolation of epithelial cells. The antibody reacts with purified native s1-m1 VacA but not with denatured VacA. The antibody recognizes a conformational epitope in the carboxy terminal portion of VacA (amino acids 685 to 821). The antibody can be used for ELISA and immunoprecipitation applications. |
| Derivation： | Animals were immunized with s1-m1 purified vacuolating cytotoxin (VacA) from Helicobacter pylori strain 60190 (ATCC 49503). VacA is a secreted protein that induces vacuolation of epithelial cells. Spleen cells were fused with Sp2/0-Ag14 cells. The antibody reacts with purified native s1-m1 VacA but not with denatured VacA. The antibody recognizes a conformational epitope in the carboxy terminal portion of VacA (amino acids 685 to 821). It does not recognize type s2-m2 VacA. |
| Genes Expressed： | immunoglobulin; monoclonal antibody; against VacA |
| Cellular Products： | immunoglobulin; monoclonal antibody; against VacA |
| Tumorigenic： | Yes |
| Effects： | Yes, forms ascites in pristane primed BALB/c mice |
| Comments： | Animals were immunized with s1-m1 purified vacuolating cytotoxin (VacA) from Helicobacter pylori strain 60190 (ATCC 49503). VacA is a secreted protein that induces vacuolation of epithelial cells. Spleen cells were fused with Sp2/0-Ag14 cells. The antibody reacts with purified native s1-m1 VacA but not with denatured VacA. The antibody recognizes a conformational epitope in the carboxy terminal portion of VacA (amino acids 685 to 821). It does not recognize type s2-m2 VacA. The antibody can be used for ELISA and immunoprecipitation applications. |
| Complete Growth Medium： | The base medium for this cell line is ATCC-formulated Dulbeccos Modified Eagles Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
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| Subculturing： | Medium Renewal: Add fresh medium every 2 to 3 days (depending on cell density) Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 2 X 10 exp5 viable cells/ml. Maintain cell density between 2 X 10 exp5 and 2 X 10 exp6 viable cells/ml. |
| Cryopreservation： | culture medium 95%; DMSO, 5% |
| Culture Conditions： | Temperature: 37.0°C |
| Isotype： | IgG1; kappa light chain |
| Name of Depositor： | TL Cover |
| Deposited As： | mouse (B cell); mouse (myeloma) |
| References： | Vinion-Dubiel AD, et al. Antigenic diversity among Helicobacter pylori vacuolating toxins. Infect. Immun. 69: 4329-4336, 2001. PubMed: 11401970 |