微信小陈
微信小章
产品简介
购买须知
| 产品名称： | 55-2 |
|---|---|
| 商品货号： | TS212828 |
| Organism： | Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma) |
| Cell Type： | hybridoma: B lymphocyte |
| Product Format： | frozen |
| Morphology： | lymphoblast |
| Culture Properties： | suspension |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Applications： | Suzanne Epstein that reacts with the gp120 of HIV-1. In ELISA the antibody reacts with rgp120 IIIB, but not with gp120 MN. It binds to the C2 region (positions 254 - 274) gp120 synthetic peptide used to map antibody reactivity, and was not able to cross block any other antibodies in the panel. |
| Derivation： | Spleen cells were fused with Sp2/0-Ag14 myeloma cells. 55-2 is one of a panel of antibodies developed by Dr. Suzanne Epstein that reacts with the gp120 of HIV-1. |
| Genes Expressed： | immunoglobulin; monoclonal antibody; against human immunodeficiency virus 1 (HIV-1) gp120 |
| Cellular Products： | immunoglobulin; monoclonal antibody; against human immunodeficiency virus 1 (HIV-1) gp120 |
| Tumorigenic： | Yes |
| Effects： | Yes, in BALB/c mice |
| Comments： | Animals were sequentially immunized with recombinant gp120 (rgp120) from three different nonglycosylated isolates of HIV-1 (IIIB, SF2, and Z6). Spleen cells were fused with Sp2/0-Ag14 myeloma cells. 55-2 is one of a panel of antibodies developed by Dr. Suzanne Epstein that reacts with the gp120 of HIV-1. In ELISA the antibody reacts with rgp120 IIIB, but not with gp120 MN. It binds to the C2 region (positions 254 - 274) gp120 synthetic peptide used to map antibody reactivity, and was not able to cross block any other antibodies in the panel. |
| Complete Growth Medium： | The base medium for this cell line is ATCC Hybri-Care Medium, Catalog No. 46-X. Hybri-Care Medium is supplied as a powder and should be reconstituted in 1 L cell-culture-grade water and supplemented with 1.5 g/L sodium bicarbonate. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
|
| Subculturing： | Medium Renewal: Every 2 to 3 days Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml. |
| Cryopreservation： | Culture medium, 95%; DMSO, 5% |
| Isotype： | IgG1 |
| Name of Depositor： | SL Epstein |
| Deposited As： | mouse (B cell); mouse (myeloma) |
| References： | Reeves JP, et al. Mouse monoclonal antibodies to human immunodeficiency virus glycoprotein 120 generated by repeated immunization with glycoprotein 120 from a single isolate, or by sequential immunization with glycoprotein 120 from three isolates. Hybridoma 14: 235-242, 1995. PubMed: 7590785 |