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| 产品名称： | 5/9 m alpha3-18 |
|---|---|
| 商品货号： | TS212838 |
| Organism： | Cricetulus griseus, hamster, Chinese |
| Tissue： | ovary |
| Product Format： | frozen |
| Morphology： | epithelial |
| Culture Properties： | adherent |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Gender： | female |
| Storage Conditions： | liquid nitrogen vapor phase |
| Disclosure： | This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC. |
| Derivation： | This line was derived from the CHO DUKX cell line. |
| Clinical Data： | female |
| Genes Expressed： | human colony stimulating factor (M-CSF) |
| Cellular Products： | human colony stimulating factor (M-CSF) |
| Comments： | The cells are resistant to methotrexate. |
| Complete Growth Medium： | Alpha minimum essential medium with 2 mM L-glutamine and 1 mM sodium pyruvate without ribonucleosides and deoxyribonucleosides supplemented with 200 nM MTX (Methotrexate) and 10% dialyzed fetal bovine serum (Sigma F0392) |
| Subculturing： |
Subcultivation Ratio: A subcultivation ratio of 1:10 to 1:20 is recommended
Medium Renewal: Two to three times weely |
| Cryopreservation： | Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO Storage temperature: liquid nitrogen vapor phase |
| Culture Conditions： | Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37°C |
| Name of Depositor： | Genetics Institute, Inc. |
| Deposited As： | Cricetulus griseus |
| U.S. Patent Number： | |
| References： | Clark SC, Wong GG. Hematopoietic growth factors. US Patent 4,868,119 dated Sep 19 1989 |