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| 产品名称： | 51.1 |
|---|---|
| 商品货号： | TS212840 |
| Organism： | Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma) |
| Cell Type： | hybridoma: B lymphocyte |
| Product Format： | frozen |
| Morphology： | lymphoblast |
| Culture Properties： | suspension |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Applications： | The antibody reacts with an antigen associated with human cytotoxic/suppressor T cells (CD8). The antibody strongly inhibits cytotoxic T lymphocyte function and cross-reacts with peripheral blood T cells from chimpanzee, orangutan, gibbon, baboon, macaque, capuchin monkey, squirrel monkey and spider monkey. |
| Derivation： | Spleen cells were fused with NSI/1 myeloma cells. |
| Genes Expressed： | immunoglobulin; monoclonal antibody; against human CD8 |
| Cellular Products： | immunoglobulin; monoclonal antibody; against human CD8 |
| Comments： | Animals were immunized with human peripheral blood T lymphocytes. Spleen cells were fused with NSI/1 myeloma cells. The antibody reacts with an antigen associated with human cytotoxic/suppressor T cells (CD8). The antibody strongly inhibits cytotoxic T lymphocyte function and cross-reacts with peripheral blood T cells from chimpanzee, orangutan, gibbon, baboon, macaque, capuchin monkey, squirrel monkey and spider monkey. |
| Complete Growth Medium： | Culture Medium: RPMI medium 1640 with 2 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate, 4.5 g/L glucose, 10 mM HEPES, and 1.0 mM sodium pyruvate suppelmented with 50 ?M 2-mercaptoethanol, 90%; fetal bovine serum, 10%. |
| Subculturing： | Medium Renewal: Every 2 to 3 days Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 1 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 5 X 10 exp5 cells/ml. |
| Isotype： | IgG2a |
| Name of Depositor： | PJ Martin |
| Deposited As： | mouse (B cell); mouse (myeloma) |
| References： | Martin PJ, et al. Epitope mapping of the human surface suppressor/cytotoxic T cell molecule Tp32. J. Immunol. 132: 759-765, 1984. PubMed: 6197474 |