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微信小章| 产品名称： | 4B2 |
|---|---|
| 商品货号： | TS212850 |
| Organism： | Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma) |
| Cell Type： | hybridoma: B lymphocyte |
| Product Format： | frozen |
| Morphology： | lymphoblast |
| Culture Properties： | suspension |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Applications： | The antibody reacts with all types of human leukocytes, but not with non-leukocytes. The antibody binds to protein A. |
| Storage Conditions： | liquid nitrogen vapor phase |
| Derivation： | Animals were immunized with human T cells.
Spleen cells were fused with P3U1 myeloma cells. |
| Genes Expressed： | immunoglobulin; monoclonal antibody; against the human T200 common leukocyte antigen (CD45) |
| Cellular Products： | immunoglobulin; monoclonal antibody; against the human T200 common leukocyte antigen (CD45) |
| Tumorigenic： | Yes, in irradiated pristane primed (BALB/c X DBA/2)F1 mice. |
| Comments： | The antibody reacts with all types of human leukocytes, but not with non-leukocytes. The antibody binds to protein A. Tested and found negative for ectromelia virus (mousepox). |
| Complete Growth Medium： | The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
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| Subculturing： | Cultures can be maintained by addition of fresh medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 1 x 105xa0viable cells/mL. Maintain cultures at a cell concentration between 2 x 105 and 1 x 106xa0cells/mL.
Medium Renewal: Every 2 to 3 days |
| Cryopreservation： | Complete growth medium described above supplemented with 5% (v/v) DMSO. Cell culture tested DMSO is available as ATCC® Catalog No. 4-X. |
| Culture Conditions： | Temperature: 37°C |
| Isotype： | IgG2a |
| Name of Depositor： | RM Steinman |
| Deposited As： | mouse (B cell); mouse (myeloma) |
| References： | . Leucocyte typing III: white cell differentiation antigens. Oxford: Oxford Univ. Press; 1987. Hay, R. J., Caputo, J. L., and Macy, M. L., Eds. (1992), ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC. Caputo, J. L., Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988. Fleming, D.O., Richardson, J. H., Tulis, J.J. and Vesley, D., (1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC. |