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微信小章| 产品名称： | 4C9 |
|---|---|
| 商品货号： | TS212854 |
| Organism： | Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma) |
| Cell Type： | hybridoma: B lymphocyte |
| Product Format： | frozen |
| Morphology： | lymphoblast |
| Culture Properties： | suspension |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Applications： | The cell line was established by Louis Gastinel and Pamela Bjorkman in 1992. Spleen cells were fused with HL-1 Friendly myeloma cells (a derivative of P3X63Ag8.653 that is deficient in both hypoxanthine phosphoribosyl transferase HPRT and adenine phosphoribosyl transferase APRT). FcRn is a heterodimer composed of a membrane bound heavy chain attached noncovalently to beta 2-microgloublin. This antibody is used in studies of functions of the MHC class I light chain. |
| Storage Conditions： | liquid nitrogen vapor phase |
| Derivation： | The cell line was established by Louis Gastinel and Pamela Bjorkman in 1992. Animals were immunized with and twice boosted with soluble neonatal Fc receptor (FcRn) in an adjuvant. Three days before fusion one mouse was boosted with purified FcRn. Spleen cells were fused with HL-1 Friendly myeloma cells (a derivative of P3X63Ag8.653 that is deficient in both hypoxanthine phosphoribosyl transferase HPRT and adenine phosphoribosyl transferase APRT). |
| Genes Expressed： | immunoglobulin; monoclonal antibody; against rat beta2 microglobulin |
| Cellular Products： | immunoglobulin; monoclonal antibody; against rat beta2 microglobulin |
| Comments： | The cell line was established by Louis Gastinel and Pamela Bjorkman in 1992. Animals were immunized with and twice boosted with soluble neonatal Fc receptor (FcRn) in an adjuvant. Three days before fusion one mouse was boosted with purified FcRn. Spleen cells were fused with HL-1 Friendly myeloma cells (a derivative of P3X63Ag8.653 that is deficient in both hypoxanthine phosphoribosyl transferase HPRT and adenine phosphoribosyl transferase APRT). The antibody recognizes rat beta 2-microglobulin alone or complexed with FcRn heavy chains. FcRn is a heterodimer composed of a membrane bound heavy chain attached noncovalently to beta 2-microgloublin. It is structurally similar to class I major histocompatibility (MHC) molecules. This antibody is used in studies of functions of the MHC class I light chain. |
| Complete Growth Medium： | HL-1 medium supplemented with 4 mM L-glutamine, 1 mM sodium pyruvate and 1% fetal bovine serum. HL-1 medium can be obtained from from Lonza (catalog number 77201).
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| Subculturing： | Subcultures are prepared by scraping. Dislodge cells from the flask substrate with a cell scraper. Aspirate gently and dispense into new flasks. Seed flasks at 2xa0x 105 viable cells/mL. Subculture Ratio:xa01:5 every 3xa0to 4 days. Medium Renewal: Add fresh medium every 2 to 3 days (depending on cell density |
| Cryopreservation： | HL-1 Complete Serum-free medium described above supplemented with 20% (v/v) fetal bovine serum and 5% (v/v) DMSO. Cell culture tested DMSO is available as ATCC Catalog No. 4-X. |
| Culture Conditions： | Temperature: 37°C
Atmosphere: Air, 95%; Carbon dioxide (CO2), 5% |
| Isotype： | IgG1 |
| Name of Depositor： | PJ Bjorkman |
| Deposited As： | mouse (B cell); mouse (myeloma) |
| Year of Origin： | 1992 |
| References： | Raghavan M, et al. Investigation of the interaction between the class I MHC-related Fc receptor and its immunoglobulin G ligand. Immunity 1: 303-315, 1994. PubMed: 7889418 Hay, R. J., Caputo, J. L., and Macy, M. L., Eds. (1992), ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC. Caputo, J. L., Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988. Fleming, D.O., Richardson, J. H., Tulis, J.J. and Vesley, D., (1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC. |