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| 产品名称： | 4D9D4 |
|---|---|
| 商品货号： | TS212856 |
| Organism： | Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma) |
| Cell Type： | hybridoma: B lymphocyte |
| Product Format： | frozen |
| Morphology： | lymphoblast |
| Culture Properties： | suspension |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Applications： | Animals were immunized with Drosophila invected protein produced in E. coli. The antibody cross-reacts with engrailed protein of Drosophila, grasshopper, leech, chicken, zebra fish, lobster and crayfish. Tested and found negative for ectromelia virus (mousepox). |
| Derivation： | Animals were immunized with Drosophila invected protein produced in E. coli. Spleen cells were fused with NS-1 myeloma cells. |
| Genes Expressed： | immunoglobulin; monoclonal antibody; against the invected and engrailed proteins of Drosophila melanogaster |
| Cellular Products： | immunoglobulin; monoclonal antibody; against the invected and engrailed proteins of Drosophila melanogaster |
| Comments： | Animals were immunized with Drosophila invected protein produced in E. coli. Spleen cells were fused with NS-1 myeloma cells. The antibody cross-reacts with engrailed protein of Drosophila, grasshopper, leech, chicken, zebra fish, lobster and crayfish. It does not recognize mouse (En-1) or sea urchin engrailed proteins. The epitope recognized by the antibody is within the homeodomain. Tested and found negative for ectromelia virus (mousepox). |
| Complete Growth Medium： | The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
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| Subculturing： | Medium Renewal: Every 2 to 3 days Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml. |
| Isotype： | IgG1 |
| Name of Depositor： | TB Kornberg |
| Deposited As： | mouse (B cell); mouse (myeloma) |
| References： | Patel NH, et al. Expression of engrailed proteins in arthropods, annelids, and chordates. Cell 58: 955-968, 1989. PubMed: 2570637 |