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| 产品名称： | 3H5-1 |
|---|---|
| 商品货号： | TS212868 |
| Organism： | Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma) |
| Cell Type： | hybridoma: B lymphocyte |
| Product Format： | frozen |
| Morphology： | lymphoblast |
| Culture Properties： | suspension |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease： | Dengue |
| Applications： | Animals were immunized with dengue virus type 2 antigens from infected mouse brains (the prototype strain New Guinea C was used). The antibody is type specific. Tested and found negative for ectromelia virus (mousepox). |
| Derivation： | Spleen cells were fused with P3X63Ag8 myeloma cells. |
| Genes Expressed： | immunoglobulin; monoclonal antibody; against a type specific determinant on Dengue virus-2) |
| Cellular Products： | immunoglobulin; monoclonal antibody; against a type specific determinant on Dengue virus-2) |
| Tumorigenic： | Yes |
| Effects： | Yes, in BALB/c mice |
| Comments： | Animals were immunized with dengue virus type 2 antigens from infected mouse brains (the prototype strain New Guinea C was used). The antibody is type specific. Spleen cells were fused with P3X63Ag8 myeloma cells. Antibodies should be prepared as ascites in pristane primed BALB/c mice. Tested and found negative for ectromelia virus (mousepox). |
| Complete Growth Medium： | The base medium for this cell line is ATCC Hybri-Care Medium, Catalog No. 46-X. Hybri-Care Medium is supplied as a powder and should be reconstituted in 1 L cell culture grade water. To make the complete growth medium, add the following components to the base medium: |
| Subculturing： | Medium Renewal: Every 2 to 3 days Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml. |
| Cryopreservation： | Freeze medium: complete growth medium, 95%; DMSO, 5% |
| Isotype： | IgG1 |
| Name of Depositor： | MK Gentry |
| Deposited As： | mouse (B cell); mouse (myeloma) |
| References： | Henchal EA, et al. Dengue virus-specific and Flavivirus group determinants identified with monoclonal antibodies by indirect immunofluorescence. Am. J. Trop. Med. Hyg. 31: 830-836, 1982. PubMed: 6285749 |