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| 产品名称： | 411-14E10 |
|---|---|
| 商品货号： | TS212869 |
| Organism： | Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma) |
| Cell Type： | hybridoma: B lymphocyte |
| Product Format： | frozen |
| Morphology： | lymphoblast |
| Culture Properties： | suspension |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Applications： | Animals were immunized with a synthetic polypeptide with the sequence SGFNADYEASSRC corresponding to peptides 4 to 17 of the fos oncogene (Note: There is a serine deletion at position 15 of the peptide antigen). In immunoblot testing, a protein of approximately 55 kD is detected in mink cells transformed by FeSV (MSTF). |
| Derivation： | Spleen cells were fused with Sp2/0 myeloma cells. |
| Genes Expressed： | immunoglobulin; monoclonal antibody; against a synthetic fos oncogene peptide |
| Cellular Products： | immunoglobulin; monoclonal antibody; against a synthetic fos oncogene peptide |
| Comments： | Animals were immunized with a synthetic polypeptide with the sequence SGFNADYEASSRC corresponding to peptides 4 to 17 of the fos oncogene (Note: There is a serine deletion at position 15 of the peptide antigen). Spleen cells were fused with Sp2/0 myeloma cells. In immunoblot testing, a protein of approximately 55 kD is detected in mink cells transformed by FeSV (MSTF). In HeLa cell nuclei (induced by TPA), a protein of approximately 40 kD is detected. |
| Complete Growth Medium： | The base medium for this cell line is ATCC-formulated Dulbeccos Modified Eagles Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
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| Subculturing： | Medium Renewal: Add fresh medium every 2 to 3 days (depending on cell density) Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 1 to 2 X 10 exp5 viable cells/ml. Maintain cell density between 1 X 10 exp5 and 1 X 10 exp6 viable cells/ml. |
| Cryopreservation： | Dulbeccos modified Eagles medium, 45%; fetal bovine serum, 45%; DMSO, 10% |
| Culture Conditions： | Temperature: 37.0°C |
| Isotype： | IgG2b; kappa light chain |
| Name of Depositor： | National Cancer Institute |
| Deposited As： | mouse (B cell); mouse (myeloma) |
| References： | Flanagan-Cato LM, McEwen BS. Pattern of Fos and Jun expression in the female rat forebrain after sexual behavior. Brain Res. 673: 53-60, 1995. PubMed: 7757479 |