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微信小章| 产品名称： | 4/4 R.M.-4 |
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| 商品货号： | TS212870 |
| Organism： | Rattus norvegicus, rat |
| Tissue： | visceral pleura |
| Product Format： | frozen |
| Morphology： | epithelial |
| Culture Properties： | adherent |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease： | normal |
| Age： | 2 to 3 months |
| Gender： | female |
| Storage Conditions： | liquid nitrogen vapor phase |
| Karyotype： | Chromosome Frequency Distribution 48 Cells: 2n = 42. Female rat karyotype with a stemline chromosome number of 42. A member of chromosome number 1 in the A group exhibited an extra fragment of chromosome attached to the long arm in all the cells examined. |
| Complete Growth Medium： | Hams F12K medium with 2 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate, 85%; fetal bovine serum, 15%
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| Subculturing： | Volumes used in this protocol are for 75 cm2 flask; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:5 is recommended Note: For more information on enzymatic dissociation and subculturing of cell lines consult Chapter 10 in Culture of Animal Cells, a manual of Basic Technique by R. Ian Freshney, 3rd edition, published by Alan R. Liss, N.Y., 1994. |
| Cryopreservation： | Culture medium 95%; DMSO, 5%. Cell culture tested DMSO is available as ATCC Catalog No. 4-X. |
| Culture Conditions： | Temperature: 37°C |
| Name of Depositor： | JF Aronson |
| Deposited As： | Rattus sp. |
| References： | Aronson JF, Cristofalo VJ. Growth of epithelial cells from rat pleura. In Vitro 15: 214, 1979. Aronson JF, Cristofalo VJ. Culture of epithelial cells from the rat pleura. In Vitro 17: 61-70, 1981. PubMed: 7216240 Hay, R. J., Caputo, J. L., and Macy, M. L., Eds. (1992), ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC. Caputo, J. L., Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988. Fleming, D.O., Richardson, J. H., Tulis, J.J. and Vesley, D., (1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC. |