微信小陈
微信小章| 产品名称： | 3T3-Swiss albino |
|---|---|
| 商品货号： | TS212873 |
| Organism： | Mus musculus, mouse |
| Tissue： | embryo |
| Cell Type： | fibroblast |
| Product Format： | frozen |
| Morphology： | fibroblast |
| Culture Properties： | adherent |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Age： | embryo |
| Storage Conditions： | liquid nitrogen vapor phase |
| Karyotype： | This is a hypertriploid mouse cell line. The modal chromosome number was 68 occurring in 30% of cells. The rate of cells with higher ploidies was 2.4%. |
| Images： |  |
| Derivation： | The 3T3 cell line was established by G. Todaro and H. Green in 1962 from disaggregated Swiss mouse embryos. |
| Genes Expressed： | Lysophosphatidylcholine (lyso-PC) induces AP-1 activity and c-jun N-terminal kinase activity (JNK1) by a protein kinase C-independent pathway.xa0Ref   Fang X, et al. Lysophosphatidylcholine stimulates activator protein 1 and the c-Jun N-terminal kinase activity. J. Biol. Chem. 272: 13683-13689, 1997. PubMed: 9153219 |
| Cellular Products： | Lysophosphatidylcholine (lyso-PC) induces AP-1 activity and c-jun N-terminal kinase activity (JNK1) by a protein kinase C-independent pathway
Ref Fang X, et al. Lysophosphatidylcholine stimulates activator protein 1 and the c-Jun N-terminal kinase activity. J. Biol. Chem. 272: 13683-13689, 1997. PubMed: 9153219 |
| Virus Susceptibility： | polyomavirus
SV40 virus |
| Comments： | The cells are contact inhibited.
A confluent monolayer yields 40000 cells/cm2.
Tested and found negative for ectromelia virus (mousepox).
The cells should be grown in plastic flasks, they do not grow well on some types of glass surfaces.
A saturation density of approximately 50000 cells/cm2 can be reached. |
| Complete Growth Medium： | The base medium for this cell line is ATCC-formulated Dulbeccos Modified Eagles Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: bovine calf serum to a final concentration of 10%.
|
| Subculturing： | Never allow culture to become completely confluent. Subculture when 80% confluent or less. Volumes used in this protocol are for 75 cm2 flask; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes.
Medium Renewal: Twice per week |
| Cryopreservation： | Freeze medium: Complete growth medium 95%; DMSO, 5% Storage temperature: liquid nitrogen vapor temperature |
| Culture Conditions： | Temperature: 37°C |
| Population Doubling Time： | 18 hrs |
| Name of Depositor： | H Green |
| Deposited As： | Mus musculus |
| Year of Origin： | 1962 |
| References： | Todaro GJ, Green H. Quantitative studies of the growth of mouse embryo cells in culture and their development into established lines. J. Cell Biol. 17: 299-313, 1963. PubMed: 13985244 Bennicelli JL, et al. Mechanism for transcriptional gain of function resulting from chromosomal translocation in alveolar rhabdomyosarcoma. Proc. Natl. Acad. Sci. USA 93: 5455-5459, 1996. PubMed: 8643596 Vogt M, Dulbecco R. Studies on cells rendered neoplastic by polyoma virus: the problem of the presence of virus-related materials. Virology 16: 41-51, 1962. PubMed: 13926482 Todaro GJ, et al. Antigenic and cultural properties of cells doubly transformed by polyoma virus and SV40. Virology 27: 179-185, 1965. PubMed: 4284655 Todaro GJ, et al. Transformation of properties of an established cell line by SV40 and polyoma virus. Proc. Natl. Acad. Sci. USA 51: 66-73, 1964. PubMed: 14104605 Fang X, et al. Lysophosphatidylcholine stimulates activator protein 1 and the c-Jun N-terminal kinase activity. J. Biol. Chem. 272: 13683-13689, 1997. PubMed: 9153219 Chen ST, et al. Generation of packaging cell lines for pseudotyped retroviral vectors of the G protein of vesicular stomatitiis virus by using a modified tetracycline inducible system. Proc. Natl. Acad. Sci. USA 93: 10057-10062, 1996. PubMed: 8816750 Campbell M, et al. The simian foamy virus type 1 transcriptional transactivator (Tas) binds and activates an enhancer element in the gag gene. J. Virol. 70: 6847-6855, 1996. PubMed: 8794326 Hsu DK, et al. Identification of a murine TEF-1-related gene expressed after mitogenic stimulation of quiescent fibroblasts and during myogenic differentiation. J. Biol. Chem. 271: 13786-13795, 1996. PubMed: 8662936 |
| Cross References： | Nucleotide (GenBank) : X85983 M.musculus mRNA for carnitine acetyltransferase. Nucleotide (GenBank) : U85711 Mus musculus phospholipase C delta-1 mRNA, complete cds. Nucleotide (GenBank) : U85713 Mus musculus phospholipase C-beta-1b mRNA, complete cds. Nucleotide (GenBank) : U85714 Mus musculus phospholipase C-beta-1b mRNA, complete cds. |