微信小陈
微信小章| 产品名称： | 3C9-D11-H11 |
|---|---|
| 商品货号： | TS212878 |
| Organism： | Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma) |
| Cell Type： | hybridoma: B lymphocyte |
| Product Format： | frozen |
| Morphology： | lymphoblast |
| Culture Properties： | suspension |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Applications： | Tested and found negative for ectromelia virus (mousepox). |
| Storage Conditions： | liquid nitrogen vapor phase |
| Derivation： | Mice were immunized with purified PPV. Spleen cells were fused with Sp2/0-Ag14 myeloma cells. |
| Genes Expressed： | immunoglobulin; monoclonal antibody; against porcine parvovirus (PPV) |
| Cellular Products： | immunoglobulin; monoclonal antibody; against porcine parvovirus (PPV) |
| Tumorigenic： | Yes |
| Effects： | Yes, in BALB/c mice |
| Comments： | Mice were immunized with purified PPV. Spleen cells were fused with Sp2/0-Ag14 myeloma cells. The antibody has both virus neutralization and hemagglutinating activities. Tested and found negative for ectromelia virus (mousepox). |
| Complete Growth Medium： | Dulbeccos modified Eagles medium with 4.5 g/L glucose, 92.5%; horse serum, 5%; bovine calf serum, 2.5%
|
| Subculturing： | Cultures can be maintained by addition of fresh medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 2 x 105 viable cells/mL.xa0 Maintain cultures at a cell concentration between 1 x 105 and 1 x 106 cells/mL. Medium Renewal: Two to three times weekly |
| Cryopreservation： | Complete growth medium described above supplemented with 5% (v/v) DMSO.xa0 Cell culture tested DMSO is available as ATCC Catalog No. 4-X. |
| Culture Conditions： | Temperature: 37°C
Atmosphere: Air, 95%; Carbon dioxide (CO2), 5% |
| Isotype： | IgG1 |
| Name of Depositor： | RA Van Deusen |
| Deposited As： | mouse (B cell); mouse (myeloma) |
| References： | 8th Proc. Intl. Pig. Vet. Soc. Gent, Belgium: 1984. Katz JB, van Deusen RA. Radioimmunoassay of adjuvant-associated porcine parvovirus using a monoclonal antibody in a nitrocellulose membrane system. J. Virol. Methods 12: 193-198, 1985. PubMed: 3009510 Hay, R. J., Caputo, J. L., and Macy, M. L., Eds. (1992), ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC. Caputo, J. L., Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988. Fleming, D.O., Richardson, J. H., Tulis, J.J. and Vesley, D., (1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC. |