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微信小章| 产品名称： | 38-1 |
|---|---|
| 商品货号： | TS212888 |
| Organism： | Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma) |
| Cell Type： | hybridoma: B lymphocyte |
| Product Format： | frozen |
| Morphology： | lymphoblast |
| Culture Properties： | suspension |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Applications： | The antibody reacts with a poorly conserved epitope on CB4 and neutralizes a restricted set of human CB4 isolates. Tested and found negative for ectromelia virus (mousepox). |
| Derivation： | Spleen cells were fused with P3X63Ag8.653 myeloma cells. |
| Genes Expressed： | immunoglobulin; monoclonal antibody; against coxsackievirus B4 (CB4); neutralizing |
| Cellular Products： | immunoglobulin; monoclonal antibody; against coxsackievirus B4 (CB4); neutralizing |
| Tumorigenic： | Yes |
| Effects： | Yes, in BALB/c mice |
| Comments： | Animals were immunized with coxsackievirus B4 (JVB strain, ATCC VR 184). Spleen cells were fused with P3X63Ag8.653 myeloma cells. The antibody reacts with a poorly conserved epitope on CB4 and neutralizes a restricted set of human CB4 isolates. Tested and found negative for ectromelia virus (mousepox). |
| Complete Growth Medium： | Culture Medium: Dulbeccos modified Eagles medium (containing 1 mM sodium pyruvate) with 4.5 g/L glucose, 90%; fetal bovine serum, 10%. |
| Subculturing： | Medium Renewal: Every 2 to 3 days Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml. |
| Isotype： | IgG2a; kappa light chain |
| Name of Depositor： | BS Prabhakar |
| Deposited As： | mouse (B cell); mouse (myeloma) |
| References： | Prabhaker BS, et al. High frequency of antigenic variants among naturally occurring human Coxsackie B4 virus isolates identified by monoclonal antibodies. Nature 300: 374-376, 1982. PubMed: 6183593 Prabhaker BS, et al. Detection of conserved and nonconserved epitopes on Coxsackievirus B4: frequency of antigenic change. Virology 146: 302-306, 1985. PubMed: 2413617 Webb SR, et al. Neutralization epitope diversity of Coxsackievirus B4 isolates detected by monoclonal antibodies. J. Med. Virol. 20: 9-15, 1986. PubMed: 2428930 |