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| 产品名称： | 38.1 |
|---|---|
| 商品货号： | TS212891 |
| Organism： | Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma) |
| Cell Type： | hybridoma: B lymphocyte |
| Product Format： | frozen |
| Morphology： | lymphoblast |
| Culture Properties： | suspension |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Applications： | The antibody reacts with the Tp19-29 antigen associated with human T cells (CD3). |
| Derivation： | Spleen cells were fused with NSI/1 myeloma cells. |
| Genes Expressed： | immunoglobulin; monoclonal antibody; against human CD3 |
| Cellular Products： | immunoglobulin; monoclonal antibody; against human CD3 |
| Comments： | Animals were immunized with human peripheral blood T lymphocytes. Spleen cells were fused with NSI/1 myeloma cells. The antibody reacts with the Tp19-29 antigen associated with human T cells (CD3). The antibody moderately inhibits alloreactive cytotoxic T lymphocyte activity and is weakly mitogenic for peripheral blood lymphocytes. |
| Complete Growth Medium： | The base medium for this cell line is ATCC Hybri-Care Medium, Catalog No. 46-X. Hybri-Care Medium is supplied as a powder and should be reconstituted in 1 L cell culture grade water. To make the complete growth medium, add the following components to the base medium: |
| Subculturing： | Medium Renewal: Every 2 to 3 days Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 1 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 5 X 10 exp5 cells/ml. |
| Isotype： | IgM |
| Name of Depositor： | PJ Martin |
| Deposited As： | mouse (B cell); mouse (myeloma) |
| References： | . Leucocyte typing: human leucocyte differentiation antig ens detected by monoclonal antibodies. New York: Springer-Verlag; 1984. |