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微信小章| 产品名称： | 3A(tPA-30-1) |
|---|---|
| 商品货号： | TS212892 |
| Organism： | Homo sapiens, human |
| Tissue： | placenta |
| Cell Type： | SV40 transformed |
| Product Format： | frozen |
| Morphology： | epithelial |
| Culture Properties： | adherent |
| Biosafety Level： | 2 xa0Cells contain polyomavirus DNA sequences
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Applications： | The cells express the transformed phenotype at the permissive temperature (33C) and the non-transformed phenotype at the non-permissive temperature (40C). |
| Storage Conditions： | liquid nitrogen vapor phase |
| Karyotype： | This is a hypodiploid human cell line with the modal chromosome number of 42 occurring in 34% of cells. However, cells with 44 chromosomes also occurred at a high frequency (26%). Most cells contained 41-45 chromosomes. Cells having a higher ploidy occurred at 20.7%, which is very high. Most cells averaged five or six marker chromosomes, none of which appeared twice in the cells examined. No chromosomes were paired consistently. |
| Genes Expressed： | At 40°C the cells synthesize human chorionic gonadotropin (hCG) and alkaline phosphatase |
| Cellular Products： | at 40C the cells synthesize human chorionic gonadotropin (hCG) and alkaline phosphatase |
| Comments： | The cells are transformed by SV40 ts30. The cells express the transformed phenotype at the permissive temperature (33C) and the non-transformed phenotype at the non-permissive temperature (40C). The line has a limited life expectancy of 15 to 18 passages before entering the crisis stage. |
| Complete Growth Medium： | Alpha minimum essential medium without ribonucleosides and deoxyribonucleosides, 90%; fetal bovine serum, 10%
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| Subculturing： | Volumes used in this protocol are for 75 cm2 flask; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes.
Subcultivation Ratio: 1:2 to 1:4 Note: For more information on enzymatic dissociation and subculturing of cell lines consult Chapter 10 in Culture of Animal Cells, a Manual of Basic Technique by R. Ian Freshney, 3rd edition, published by Alan R. Liss, N.Y., 1994. |
| Cryopreservation： | Complete growth medium described above supplemented with 5% (v/v) DMSO.xa0 Cell culture tested DMSO is available as ATCC Catalog No. 4-X. |
| Culture Conditions： | Temperature: 37°C
Atmosphere: Air, 95%; Carbon dioxide (CO2), 5% |
| STR Profile： | Amelogenin: X CSF1PO: 10,12 D13S317: 11 D16S539: 9,12 D5S818: 11,13 D7S820: 10 THO1: 9,9.3 TPOX: 8,11 vWA: 16,17 |
| Name of Depositor： | J Chou |
| Deposited As： | Homo sapiens |
| Passage History： | The line has a limited life expectancy of 15 to 18 passages before entering the crisis stage. |
| References： | Chou JY. Human placental cells transformed by tsA mutants of simian virus 40: a model system for the study of placental functions. Proc. Natl. Acad. Sci. USA 75: 1409-1413, 1978. PubMed: 206898 Hay, R. J., Caputo, J. L., and Macy, M. L., Eds. (1992), ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC. Caputo, J. L., Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988. Fleming, D.O., Richardson, J. H., Tulis, J.J. and Vesley, D., (1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC. Biosafety in Microbiological and Biomedical Laboratories, 5th ed. HHS. U.S. Department of Health and Human Services, Centers for Disease Control and Prevention. Washington DC: U.S. Government Printing Office; 2007. The entire text is available online. |