微信小陈
微信小章
产品简介
购买须知
| 产品名称： | 372 |
|---|---|
| 商品货号： | TS212894 |
| Organism： | Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma) |
| Cell Type： | hybridoma: B lymphocyte |
| Product Format： | frozen |
| Morphology： | lymphoblast |
| Culture Properties： | suspension |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease： | Leukemia |
| Applications： | Spleen cells from a mouse inoculated with a B-tropic Friend murine leukemia virus (FrMuLV) were fused with NS-1 myeloma cells. The antibody is highly specific for p15E protein from most MuLV. |
| Derivation： | Spleen cells from a mouse inoculated with a B-tropic Friend murine leukemia virus (FrMuLV) were fused with NS-1 myeloma cells. |
| Genes Expressed： | immunoglobulin; monoclonal antibody; against p15E envelop antigen (ENV) protein of murine leukemia viruses (MuLV) |
| Cellular Products： | immunoglobulin; monoclonal antibody; against p15E envelop antigen (ENV) protein of murine leukemia viruses (MuLV) |
| Comments： | Spleen cells from a mouse inoculated with a B-tropic Friend murine leukemia virus (FrMuLV) were fused with NS-1 myeloma cells. The antibody is highly specific for p15E protein from most MuLV. The antibody efficiently immunoprecipitates p15E and works well in Western blotting assays for p15E. |
| Complete Growth Medium： | RPMI 1640 medium with 0.05 mM 2-mercaptoethanol, 90%; fetal bovine serum, 10%
|
| Subculturing： | Medium Renewal: Every 2 to 3 days Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml. |
| Isotype： | IgG3 |
| Name of Depositor： | B Chesebro |
| Deposited As： | mouse (B cell); mouse (myeloma) |
| References： | Chesebro B, et al. Characterization of mouse monoclonal antibodies specific for Friend murine leukemia virus-induced erythroleukemia cells: Friend-specific and FMR-specific antigens. Virology 112: 131-144, 1981. PubMed: 6787798 Chesebro B, et al. Characterization of monoclonal antibodies reactive with murine leukemia viruses: Use in analysis of strains of Friend MCF and Friend ecotropic murine leukemia virus. Virology 127: 134-148, 1983. PubMed: 6305011 |