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微信小章| 产品名称： | 3A-sub E post crisis of 3A(tPA-30-1) |
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| 商品货号： | TS212895 |
| Organism： | Homo sapiens, human |
| Tissue： | placenta |
| Cell Type： | SV40 transformed |
| Product Format： | frozen |
| Culture Properties： | adherent |
| Biosafety Level： | 2 Cells contain polyomavirus DNA sequences
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Applications： | This line was established from ATCC CRL-1583 cells that had begun to senesce. |
| Storage Conditions： | liquid nitrogen vapor phase |
| Derivation： | This line was established from ATCC CRL-1583 cells that had begun to senesce. |
| Genes Expressed： | at 40C the cells produce human chorionic gonadotropin (hCG) |
| Cellular Products： | at 40C the cells produce human chorionic gonadotropin (hCG) |
| Comments： | This line was established from ATCC CRL-1583 cells that had begun to senesce. By passage 20 the cells had recovered and appeared capable of unlimited proliferation. The saturation density (6 X 10 exp5 cells/sq cm) is increased and the morphology is altered relative to the parental line. |
| Complete Growth Medium： | The base medium for this cell line is ATCC-formulated Eagles Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
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| Subculturing： | Volumes used in this protocol are for 75 cm2 flask; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes.
Subcultivation Ratio: 1:2 Note: For more information on enzymatic dissociation and subculturing of cell lines consult Chapter 10 in Culture of Animal Cells, a Manual of Basic Technique by R. Ian Freshney, 3rd edition, published by Alan R. Liss, N.Y., 1994. |
| Cryopreservation： | Complete growth medium described above supplemented with 5% (v/v) DMSO.xa0 Cell culture tested DMSO is available as ATCC Catalog No. 4-X. |
| Culture Conditions： | Temperature: 33°C
Atmosphere: Air, 95%; Carbon dioxide (CO2), 5% |
| STR Profile： | Amelogenin: X CSF1PO: 10,12 D13S317: 11 D16S539: 9,12 D5S818: 11,13 D7S820: 10 THO1: 9,9.3 TPOX: 8,11 vWA: 16,17 |
| Name of Depositor： | MI Cour |
| Deposited As： | Homo sapiens |
| Passage History： | By passage 20 the cells had recovered and appeared capable of unlimited proliferation. |
| References： | Chou JY. Human placental cells transformed by tsA mutants of simian virus 40: a model system for the study of placental functions. Proc. Natl. Acad. Sci. USA 75: 1409-1413, 1978. PubMed: 206898 Hay, R. J., Caputo, J. L., and Macy, M. L., Eds. (1992), ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC. Caputo, J. L., Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988. Fleming, D.O., Richardson, J. H., Tulis, J.J. and Vesley, D., (1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC. Reiter J, et al. Cytogenetic features of human trophoblast cell lines SWAN-71 and 3A-subE. Placenta 52: 17-20, 2017. |