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| 产品名称： | 35.1 |
|---|---|
| 商品货号： | TS212899 |
| Organism： | Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma) |
| Cell Type： | hybridoma: B lymphocyte |
| Product Format： | frozen |
| Morphology： | lymphoblast |
| Culture Properties： | suspension |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Applications： | The antibody reacts with the human T cell surface protein Tp50 (CD2). |
| Derivation： | Spleen cells were fused with NSI/1 myeloma cells. |
| Genes Expressed： | immunoglobulin; monoclonal antibody; against human CD2 |
| Cellular Products： | immunoglobulin; monoclonal antibody; against human CD2 |
| Comments： | Animals were immunized with human T lymphocytes that had been activated with phorbol myristic acid (PMA). Spleen cells were fused with NSI/1 myeloma cells. The antibody reacts with the human T cell surface protein Tp50 (CD2). The antibody inhibits T cell proliferative responses and cytotoxicity, but does not inhibit antibody mediated cellular cytotoxicity. |
| Complete Growth Medium： | RPMI 1640 medium with 0.05 mM 2-mercaptoethanol and 2 mM L-glutamine, 90%; fetal bovine serum, 10%
|
| Subculturing： | Medium Renewal: Every 2 to 3 days Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 1 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 5 X 10 exp5 cells/ml. |
| Isotype： | IgG2a |
| Name of Depositor： | PJ Martin |
| Deposited As： | mouse (B cell); mouse (myeloma) |
| References： | Martin PJ, et al. Identification and functional characterization of two distinct epitopes on the human T cell surface protein Tp50. J. Immunol. 131: 180-185, 1983. PubMed: 6190907 |