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微信小章| 产品名称： | 36.5 |
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| 商品货号： | TS212906 |
| Organism： | Mus musculus, mouse |
| Tissue： | embryo |
| Cell Type： | pluripotent embryonic stem cell |
| Product Format： | frozen |
| Morphology： | epithelial |
| Culture Properties： | adherent |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Age： | embryo, blastocyst |
| Strain： | 129/Sv+c/+p |
| Applications： | This line was derived by inserting a construct containing a disrupted murine Lyt-2 (CD8) gene. After selection for neomycin resistance and presence of Lyt-2 sequences upstream of the insertion, the 36.5 cell line was established. This line has been used to produce mice deficient in expression of CD8. The cells can be maintained in the undifferentiated state by frequent subculture on feeder layers of irradiated (12000 rads) or mitomycin C treated (0.01 mg/ml for 90 minutes) primary mouse embryonic fibroblasts or STO cells. |
| Storage Conditions： | liquid nitrogen vapor phase |
| Disclosure： | This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC. |
| Derivation： | This line was derived by inserting a construct containing a disrupted murine Lyt-2 (CD8) gene. |
| Comments： | This line was derived by inserting a construct containing a disrupted murine Lyt-2 (CD8) gene.xa0 The Lyt-2 genes was disrupted by insertion of a plasmid containing a neomycin resistance gene into the first exon of the Lyt-2 gene.xa0 After selection for neomycin resistance and presence of Lyt-2 sequences upstream of the insertion, the 36.5 cell line was established.xa0 This line has been used to produce mice deficient in expression of CD8. The cells can be maintained in the undifferentiated state by frequent subculture on feeder layers of mitomycin C treated STO cells (MITC-STO ATCC 56-X.2) (see ATCC 56-X.2, MITC-STO cells). |
| Complete Growth Medium： | Dulbeccos modified Eagles medium with 4 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate and 4.5 g/L glucose and supplemented with 0.1 mM 2-mercaptoethanol, 85%; fetal bovine serum, 15%
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| Subculturing： | Volumes used in this protocol are for 75 cm2 flask; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes. The line should be grown on feeder layers of irradiated or mitomycin C treated (0.01 mg/mLfor 90 minutes) primary mouse embryonic fibroblasts or STO cells (see ATCC CRL-1503 or ATCC 56-X, irradiated STO cells).
Subcultivation Ratio: 1:3 to 1:6 is recommended |
| Cryopreservation： | Complete growth medium described above supplemented with 5% (v/v) DMSO.xa0 Cell culture tested DMSO is available as ATCC Catalog No. 4-X. |
| Culture Conditions： | Temperature: 37°C
Atmosphere: Air, 95%; Carbon dioxide (CO2), 5% |
| Name of Depositor： | Ontario Cancer Institute |
| Deposited As： | Mus musculus |
| U.S. Patent Number： | |
| References： | Mak TW. Mutant mouse lacking CD8 surface marker. US Patent 5,530,178 dated Jun 25 1996 Hay, R. J., Caputo, J. L., and Macy, M. L., Eds. (1992), ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC. Caputo, J. L., Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988. Fleming, D.O., Richardson, J. H., Tulis, J.J. and Vesley, D., (1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC. Biosafety in Microbiological and Biomedical Laboratories, 5th ed. HHS. U.S. Department of Health and Human Services, Centers for Disease Control and Prevention. Washington DC: U.S. Government Printing Office; 2007. The entire text is available online. |