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| 产品名称： | 36F-18C 36F |
|---|---|
| 商品货号： | TS212907 |
| Organism： | Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma) |
| Cell Type： | hybridoma: B lymphocyte |
| Product Format： | frozen |
| Morphology： | lymphoblast |
| Culture Properties： | suspension |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Applications： | The antibody is specific for sheep CD2 and precipitates a 50000 to 55000 dalton molecule from lysates of sheep lymphocytes. |
| Derivation： | Spleen cells were fused with Sp2/0-Ag14 myeloma cells. |
| Genes Expressed： | immunoglobulin; monoclonal antibody; against sheep (ovine) CD2 |
| Cellular Products： | immunoglobulin; monoclonal antibody; against sheep (ovine) CD2 |
| Comments： | Animals were immunized with phytohemagglutinin-stimulated sheep T cells. Spleen cells were fused with Sp2/0-Ag14 myeloma cells. The antibody is specific for sheep CD2 and precipitates a 50000 to 55000 dalton molecule from lysates of sheep lymphocytes. It does not inhibit cytotoxic T lymphocyte mediated killing or mixed lymphocyte reactivity. |
| Complete Growth Medium： | CULTURE MEDIUM: Iscoves modified Dulbeccos medium, 90%; fetal bovine serum, 10%. |
| Subculturing： | Medium Renewal: Every 2 to 3 days Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml. |
| Cryopreservation： | Culture medium, 95%; DMSO, 5% |
| Isotype： | IgG2a |
| Name of Depositor： | CR Mackay |
| Deposited As： | mouse (B cell); mouse (myeloma) |
| References： | Mackay CR, et al. Unusual expression of CD2 in sheep: implications for T cell interactions. Eur. J. Immunol. 18: 1681-1688, 1988. PubMed: 2462499 |