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| 产品名称： | 3197-3 (3T3-MSV) |
|---|---|
| 商品货号： | TS212910 |
| Organism： | Mus musculus, mouse |
| Tissue： | embryo |
| Cell Type： | fibroblast |
| Product Format： | frozen |
| Morphology： | fibroblast |
| Culture Properties： | adherent |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease： | sarcoma |
| Age： | embryo |
| Applications： | The cells were derived from ATCC CCL-92 by transformation with Moloney murine sarcoma virus. They do not produce virus particles. Tested and found negative for ectromelia virus (mousepox). |
| Derivation： | The cells were derived from ATCC CCL-92 by transformation with Moloney murine sarcoma virus. |
| Genes Expressed： | The cells release a growth factor in serum free medium. |
| Cellular Products： | The cells release a growth factor in serum free medium. |
| Comments： | The cells were derived from ATCC CCL-92 by transformation with Moloney murine sarcoma virus. They do not produce virus particles. The cells have a tendency to slough off the flask surface. Tested and found negative for ectromelia virus (mousepox). |
| Complete Growth Medium： | Dulbeccos modified Eagles medium, 95%; bovine calf serum, 5%
|
| Subculturing： | Subcultivation Ratio: A subcultivation ratio of 1:6 to 1:8 is recommended Medium Renewal: 1 to 2 times per week Remove medium, add fresh 0.25% trypsin solution for 5 minutes, add fresh medium, aspirate and dispense into new flasks. Subculture every 3 to 6 days. |
| Name of Depositor： | JE DeLarco |
| Deposited As： | Mus musculus |
| References： | de Larco JE, Todaro GJ. Growth factors from murine sarcoma virus-transformed cells. Proc. Natl. Acad. Sci. USA 75: 4001-4005, 1978. PubMed: 211512 Bassin RH, et al. Isolation of murine sarcoma virus-transformed mouse cells which are negative for leukemia virus from agar suspension cultures. Int. J. Cancer 6: 95-107, 1970. PubMed: 4319233 |