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| 产品名称： | 331.12 |
|---|---|
| 商品货号： | TS212911 |
| Organism： | Rattus norvegicus (B cell); Mus musculus (myeloma), rat (B cell); mouse (myeloma) |
| Cell Type： | hybridoma: B lymphocyte |
| Product Format： | frozen |
| Morphology： | lymphoblast |
| Culture Properties： | suspension |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease： | Lymphoma |
| Applications： | At the ATCC tested and found negative for ectromelia virus (mousepox). |
| Clinical Data： | The line was cured of a mycoplasma infection by treatment with BM-cycline and was recloned by the donor. |
| Genes Expressed： | immunoglobulin; monoclonal antibody; against mouse IgM (mu heavy chain) |
| Cellular Products： | immunoglobulin; monoclonal antibody; against mouse IgM (mu heavy chain) |
| Comments： | Animals were immunized with W279 B lymphoma cells. The line was cured of a mycoplasma infection by treatment with BM-cycline and was recloned by the donor. At the ATCC tested and found negative for ectromelia virus (mousepox). |
| Complete Growth Medium： | The base medium for this cell line is ATCC-formulated Dulbeccos Modified Eagles Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
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| Subculturing： | Medium Renewal: Add fresh medium every 2 to 3 days (depending on cell density) Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 x 10 exp5 cells/ml and maintain between 1 x 10 exp5 and 1 x 10 exp6 cells/ml. |
| Cryopreservation： | culture medium 95%; DMSO, 5% |
| Culture Conditions： | Temperature: 37.0°C |
| Isotype： | IgG2b |
| Name of Depositor： | PW Kincade |
| Deposited As： | rat (B cell); mouse (myeloma) |
| References： | Kincade PW, et al. Monoclonal rat antibodies to murine IgM determinants. J. Immunol. Methods 42: 17-26, 1981. PubMed: 6165776 |