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微信小章| 产品名称： | 34 |
|---|---|
| 商品货号： | TS212915 |
| Organism： | Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma) |
| Cell Type： | hybridoma: B lymphocyte |
| Product Format： | frozen |
| Morphology： | lymphoblast |
| Culture Properties： | suspension |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease： | Erythroleukemia |
| Applications： | The antibody reacts with the p15 protein from several MuLV including Friend, Moloney, Rauscher, amphotropic and Spleen Focus Forming (SFFV) viruses. The antibody reacts with infected cells after acetone fixation and immunoprecipitates p15. |
| Storage Conditions： | liquid nitrogen vapor phase |
| Derivation： | Spleen cells from a mouse recovering from erythroleukemia induced by a B-tropic Friend murine leukemia virus were fused with NS-1 myeloma cells. |
| Genes Expressed： | immunoglobulin; monoclonal antibody; against p15 group antigen (GAG) protein of murine leukemia viruses (MuLV) |
| Cellular Products： | immunoglobulin; monoclonal antibody; against p15 group antigen (GAG) protein of murine leukemia viruses (MuLV) |
| Comments： | Spleen cells from a mouse recovering from erythroleukemia induced by a B-tropic Friend murine leukemia virus were fused with NS-1 myeloma cells. The antibody reacts with the p15 protein from several MuLV including Friend, Moloney, Rauscher, amphotropic and Spleen Focus Forming (SFFV) viruses. The antibody reacts with infected cells after acetone fixation and immunoprecipitates p15. |
| Complete Growth Medium： | The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium:
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| Subculturing： | Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 x 105 cells/mL and maintain between 1 x 105 and 1 x 106 cells/mL.
Medium Renewal: Every 2 to 3 days |
| Cryopreservation： | Complete growth medium described above supplemented with 5% (v/v) DMSO.xa0 Cell culture tested DMSO is available as ATCC Catalog No. 4-X. |
| Culture Conditions： | Temperature: 37°C
Atmosphere: Air, 95%; Carbon dioxide (CO2), 5% |
| Isotype： | IgG2b , IgG2b |
| Name of Depositor： | B Chesebro |
| Deposited As： | mouse (B cell); mouse (myeloma) |
| References： | Chesebro B, et al. Characterization of mouse monoclonal antibodies specific for Friend murine leukemia virus-induced erythroleukemia cells: Friend-specific and FMR-specific antigens. Virology 112: 131-144, 1981. PubMed: 6787798 Chesebro B, et al. Characterization of monoclonal antibodies reactive with murine leukemia viruses: Use in analysis of strains of Friend MCF and Friend ecotropic murine leukemia virus. Virology 127: 134-148, 1983. PubMed: 6305011 Hay, R. J., Caputo, J. L., and Macy, M. L., Eds. (1992), ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC. Caputo, J. L., Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988. Fleming, D.O., Richardson, J. H., Tulis, J.J. and Vesley, D., (1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC. Biosafety in Microbiological and Biomedical Laboratories, 5th ed. HHS. U.S. Department of Health and Human Services, Centers for Disease Control and Prevention. Washington DC: U.S. Government Printing Office; 2007. The entire text is available online. |