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| 产品名称： | 33D1 |
|---|---|
| 商品货号： | TS212916 |
| Organism： | Rattus norvegicus (B cell); Mus musculus (myeloma), rat (B cell); mouse (myeloma) |
| Cell Type： | hybridoma: B lymphocyte |
| Product Format： | frozen |
| Morphology： | lymphoblast |
| Culture Properties： | suspension |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Applications： | Tested and found negative for ectromelia virus (mousepox). |
| Derivation： | Spleen cells were fused with P3X63Ag8-U1 myeloma cells. |
| Genes Expressed： | immunoglobulin; monoclonal antibody; against mouse dendritic cells (cytotoxic) |
| Cellular Products： | immunoglobulin; monoclonal antibody; against mouse dendritic cells (cytotoxic) |
| Comments： | Animals were immunized with mouse spleen and lymph node dendritic cells. Spleen cells were fused with P3X63Ag8-U1 myeloma cells. The antigen recognized by the 33D1 antibody appears to be present only on dendritic cells (10000 to 15000 sites/cell). The antigen does not appear to be immune response associated antigen or Ia antigen. Tested and found negative for ectromelia virus (mousepox). |
| Complete Growth Medium： | The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
|
| Subculturing： | Medium Renewal: Every 2 to 3 days Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml. |
| Isotype： | IgG2b |
| Name of Depositor： | RM Steinman |
| Deposited As： | rat (B cell); mouse (myeloma) |
| References： | Nussenzweig MC, et al. A monoclonal antibody specific for mouse dendritic cells. Proc. Natl. Acad. Sci. USA 79: 161-165, 1982. PubMed: 6948298 Steinman RM, et al. Dendritic cells are the principal stimulators of the primary mixed leukocyte reaction in mice. J. Exp. Med. 157: 613-627, 1983. PubMed: 6185614 |