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微信小章| 产品名称： | 2PK-3 |
|---|---|
| 商品货号： | TS212918 |
| Organism： | Mus musculus, mouse |
| Cell Type： | B lymphocyte |
| Product Format： | frozen |
| Morphology： | lymphoblast |
| Culture Properties： | suspension |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease： | lymphoma |
| Strain： | BALB/c |
| Storage Conditions： | liquid nitrogen vapor phase |
| Derivation： | Derived from a lymphoma induced with mineral oil and casein in a BALB/c mouse.
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| Antigen Expression： | I-A d; I-E d |
| Genes Expressed： | surface immunoglobulin (sIg +) |
| Cellular Products： | surface immunoglobulin (sIg +) |
| Comments： | It is representative of a mature B lymphocyte just prior to the plasma cell stage of differentiation. Tested and found negative for ectromelia virus (mousepox). |
| Complete Growth Medium： | The base medium for this cell line is ATCC-formulated Dulbeccos Modified Eagles Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: 2-mercaptoethanol to a final concentration of 0.05 mM; fetal bovine serum to a final concentration of 10%.
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| Subculturing： | Cultures can be maintained by addition of fresh medium. Alternatively, cultures can be subcultured by total medium replacement by centrifugation with subsequent resuspension at 1 x 105xa0viable cells/mL. Maintain cultures at a cell concentration between 1 x 105 and 1 x 106xa0cells/mL. Do not allow the cell concentration to exceed 1 x 106xa0cells/mL.
Medium Renewal: Every 2 to 3 days |
| Cryopreservation： | Complete growth medium, 95%; DMSO, 5%.xa0Cell culture tested DMSO is available as ATCC Catalog No. 4-X.
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| Culture Conditions： | Temperature: 37°C Atmosphere: 5% CO2 |
| Isotype： | IgG2 kappa , IgG2; kappa light chain |
| Name of Depositor： | NL Warner |
| Deposited As： | Mus musculus |
| References： | . Membrane receptors of lymphocytes. Amsterdam: Elsevier/North Holland; 1975. Warner NL, et al. Flow cytometry analysis of murine B cell lymphoma differentiation. Immunol. Rev. 48: 197-243, 1979. PubMed: 161906 Lanier LL, et al. Quantitative immunofluorescent analysis of surface phenotypes of murine B cell lymphomas and plasmacytomas with monoclonal antibodies. J. Immunol. 127: 1691-1697, 1981. PubMed: 7024413 Brumeanu TD, et al. Immunogenicity of a contiguous T-B synthetic epitope of the A/PR/8/34 influenza virus. J. Virol. 71: 5473-5480, 1997. PubMed: 9188620 Gutman GA, et al. Immunoglobulin production by murine B-lymphoma cells. Clin. Immunol. Immunopathol. 18: 230-244, 1981. PubMed: 6781803 Casares S, et al. Antigen presentation of dendritic cells after immunization with DNA encoding a major histocompatability complex class II-restricted viral epitope. J. Exp. Med. 186: 1481-1486, 1997. PubMed: 9348305 Hay, R. J., Caputo, J. L., and Macy, M. L., Eds. (1992), ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC. Caputo, J. L., Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988. Fleming, D.O., Richardson, J. H., Tulis, J.J. and Vesley, D., (1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC. |