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| 产品名称： | 3.155 |
|---|---|
| 商品货号： | TS212920 |
| Organism： | Rattus norvegicus (B cell); Mus musculus (myeloma), rat (B cell); mouse (myeloma) |
| Cell Type： | hybridoma: B lymphocyte |
| Product Format： | frozen |
| Morphology： | lymphoblast |
| Culture Properties： | suspension |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Applications： | Tested and found negative for ectromelia virus (mousepox). |
| Storage Conditions： | liquid nitrogen vapor phase |
| Derivation： | Spleen cells were fused with Sp2/0-Ag14 myeloma cells. |
| Genes Expressed： | immunoglobulin; monoclonal antibody; against Lyt-2 (mouse cytotoxic T cell antigen, all alleles) |
| Cellular Products： | immunoglobulin; monoclonal antibody; against Lyt-2 (mouse cytotoxic T cell antigen, all alleles) |
| Comments： | Animals were immunized with CTL Clone L3 cells. Spleen cells were fused with Sp2/0-Ag14 myeloma cells. Tested and found negative for ectromelia virus (mousepox). |
| Complete Growth Medium： | The base medium for this cell line is ATCC-formulated Dulbeccos Modified Eagles Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 20%.
|
| Subculturing： | Medium Renewal: 2 to 3 times per week Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 1 x 10 (5) cells/ml and maintain between 1 x 10 (5) and 1 x 10 (6) cells/ml. |
| Cryopreservation： | Freeze medium: complete growth medium, 95%; DMSO, 5% |
| Culture Conditions： | Temperature: 37.0°C Atmosphere: air, 95%; carbon dioxide (CO2), 5% |
| Isotype： | IgM |
| Name of Depositor： | FW Fitch |
| Deposited As： | rat (B cell); mouse (myeloma) |
| References： | Sarmiento M, et al. IgG or IgM monoclonal antibodies reactive with different determinants on the molecular complex bearing lyt 2 antigen block T-cell mediated cytolysis in the absence of complement. J. Immunol. 125: 2665-2672, 1980. PubMed: 6159417 Sarmiento M, et al. Cloned T lymphocytes and monoclonal antibodies as probes for cell surface molecules active in T cell-mediated cytolysis. Immunol. Rev. 68: 135-169, 1982. PubMed: 6184304 |