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| 产品名称： | 2H6-C2 |
|---|---|
| 商品货号： | TS212921 |
| Organism： | Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma) |
| Cell Type： | hybridoma: B lymphocyte |
| Product Format： | frozen |
| Morphology： | lymphoblast |
| Culture Properties： | suspension |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Applications： | The antibody is specific for the gD glycoprotein of BHV-1 and is used by the National Veterinary Services Laboratories of the USDA for in vitro potency testing of adjuvantated, inactivated BHV-1 vaccines. |
| Derivation： | Spleen cells were fused with Sp2/0-Ag14 myeloma cells. |
| Genes Expressed： | immunoglobulin; monoclonal antibody; against Bovine herpesvirus 1 (BHV-1, also known as infectious bovine rhinotracheitis virus (IBRV)) |
| Cellular Products： | immunoglobulin; monoclonal antibody; against Bovine herpesvirus 1 (BHV-1, also known as infectious bovine rhinotracheitis virus (IBRV)) |
| Tumorigenic： | Yes |
| Effects： | Yes, in BALB/c mice |
| Comments： | Animals were immunized with BHV-1. Spleen cells were fused with Sp2/0-Ag14 myeloma cells. The antibody is specific for the gD glycoprotein of BHV-1 and is used by the National Veterinary Services Laboratories of the USDA for in vitro potency testing of adjuvantated, inactivated BHV-1 vaccines. |
| Complete Growth Medium： | Dulbeccos modified Eagles medium with 4.5 g/L glucose, 92.5%; horse serum, 5%; fetal bovine serum, 2.5%
|
| Subculturing： | Medium Renewal: Every 2 to 3 days Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml. |
| Isotype： | IgG2b |
| Name of Depositor： | CA Whetstone |
| Deposited As： | mouse (B cell); mouse (myeloma) |
| References： | . . Conf. Res. Workers Anim. Dis. : 54, 1987. |