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| 产品名称： | 312-13E8 |
|---|---|
| 商品货号： | TS212926 |
| Organism： | Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma) |
| Cell Type： | hybridoma: B lymphocyte |
| Product Format： | frozen |
| Morphology： | lymphoblast |
| Culture Properties： | suspension |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Applications： | Animals were immunized with a synthetic peptide with the sequence LMRACWQWNPSDRPSF corresponding to residues 471-486 of the abl oncogene (formerly 590 to 605 of the v-abl oncogene). |
| Images： |  |
| Derivation： | Spleen cells were fused with Sp2/0 myeloma cells. |
| Genes Expressed： | immunoglobulin; monoclonal antibody; against a synthetic abl oncogene peptide |
| Cellular Products： | immunoglobulin; monoclonal antibody; against a synthetic abl oncogene peptide |
| Comments： | Animals were immunized with a synthetic peptide with the sequence LMRACWQWNPSDRPSF corresponding to residues 471-486 of the abl oncogene (formerly 590 to 605 of the v-abl oncogene). Spleen cells were fused with Sp2/0 myeloma cells. In ELISA testing, the antibody recognizes the fes, mos, erb B, src, fgr, yes, mil, raf, fms, and insulin receptor sequences in addition to the abl sequence. In immunoblot testing, a protein of approximately 80 kd is recognized in MOLT-4 cells. The IgG1 reactivity of the antibody with the abl peptide (471 to 486) was confirmed by ELISA. IgG1 was strongly positive while IgG2b was negative. |
| Complete Growth Medium： | The base medium for this cell line is ATCC-formulated Dulbeccos Modified Eagles Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
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| Subculturing： | Medium Renewal: Add fresh medium every 2 to 3 days (depending on cell density) Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 1 to 2 X 10 exp5 viable cells/ml. Maintain cell density between 1 X 10 exp5 and 1 X 10 exp6 viable cells/ml. |
| Cryopreservation： | culture medium 95%; DMSO, 5% |
| Culture Conditions： | Temperature: 37.0°C |
| Isotype： | IgG1 and IgG2b |
| Antigenic Determinant： | abl oncogene peptide, synthetic |
| Name of Depositor： | National Cancer Institute |
| Deposited As： | mouse (B cell); mouse (myeloma) |