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微信小章| 产品名称： | 30-H12 |
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| 商品货号： | TS212927 |
| Organism： | Rattus norvegicus (B cell); Mus musculus (myeloma), rat (B cell); mouse (myeloma) |
| Cell Type： | hybridoma: B lymphocyte |
| Product Format： | frozen |
| Morphology： | lymphoblast |
| Culture Properties： | suspension |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Storage Conditions： | liquid nitrogen vapor phase |
| Derivation： | Animals were immunized with splenic or thymic lymphocytes.
Spleen cells were fused with NS-1 myeloma cells.
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| Genes Expressed： | immunoglobulin; monoclonal antibody; against Thy-1.2; cytotoxic |
| Cellular Products： | immunoglobulin; monoclonal antibody; against Thy-1.2; cytotoxic |
| Comments： | Tested and found negative for ectromelia virus (mousepox). |
| Complete Growth Medium： | The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
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| Subculturing： | Cultures can be maintained by addition of fresh medium or by replacement of medium. Adherent cells can be dislodged by scraping, and cultures may be established by centrifugation with subsequent resuspension at 2 x 105xa0viable cells/mL. A saturation density of 1 x 106xa0cells/mL is obtainable. Medium Renewal: Every 2 to 3 days |
| Culture Conditions： | Temperature: 37°C |
| Isotype： | IgG2b |
| Name of Depositor： | JA Ledbetter |
| Deposited As： | rat (B cell); mouse (myeloma) |
| References： | Fidel PL Jr., et al. Use of cellular depletion analysis to examine circulation of immune effector function between the vagina and the periphery. Infect. Immun. 65: 3939-3943, 1997. PubMed: 9284175 Garvy BA, Harmsen AG. The role of T cells in infection-driven interstitial pneumonia after bone marrow transplantation in mice. Transplantation 62: 517-525, 1996. PubMed: 8781619 Wilson ME, et al. Local suppression of IFN-gamma in hepatic granulomas correlates with tissue-specific replication of Leishmania chagasi. J. Immunol. 156: 2231-2239, 1996. PubMed: 8690913 Sayles PC, Johnson LL. Intact immune defenses are required for mice to resist the ts-4 vaccine strain of Toxoplasma gondii. Infect. Immun. 64: 3088-3092, 1996. PubMed: 8757838 Ledbetter JA, Herzenberg LA. Xenogeneic monoclonal antibodies to mouse lymphoid differentiation antigens. Immunol. Rev. 47: 63-90, 1979. PubMed: 398327 Hay, R. J., Caputo, J. L., and Macy, M. L., Eds. (1992), ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC. Caputo, J. L., Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988. Fleming, D.O., Richardson, J. H., Tulis, J.J. and Vesley, D., (1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC. |