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| 产品名称： | 2E.6 |
|---|---|
| 商品货号： | TS212931 |
| Organism： | Rattus norvegicus (B cell); Mus musculus (myeloma), rat (B cell); mouse (myeloma) |
| Cell Type： | hybridoma: B lymphocyte |
| Product Format： | frozen |
| Morphology： | lymphoblast |
| Culture Properties： | suspension |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Applications： | Tested and found negative for ectromelia virus (mousepox). |
| Derivation： | Spleen cells were fused with Sp2/0-Ag14 myeloma cells. |
| Genes Expressed： | immunoglobulin; monoclonal antibody; against mouse IgG3 |
| Cellular Products： | immunoglobulin; monoclonal antibody; against mouse IgG3 |
| Comments： | Animals were immunized with mouse myeloma proteins of the IgG1 (MOPC-21), IgG2a (HOPC-1), IgG2b (MPC-11), IgG3 (J606 and GAC-39) and IgM (M104E) subclasses. Spleen cells were fused with Sp2/0-Ag14 myeloma cells. Tested and found negative for ectromelia virus (mousepox). |
| Complete Growth Medium： | Culture Medium: Dulbeccos modified Eagles medium with non-essential amino acids, 90%; horse serum, 10%. |
| Subculturing： | Medium Renewal: Every 2 to 3 days Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml. |
| Isotype： | IgG1 |
| Name of Depositor： | JM Davie |
| Deposited As： | rat (B cell); mouse (myeloma) |