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微信小章| 产品名称： | 2E10-H2 |
|---|---|
| 商品货号： | TS212932 |
| Organism： | Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma) |
| Cell Type： | hybridoma: B lymphocyte |
| Product Format： | frozen |
| Morphology： | lymphoblast |
| Culture Properties： | suspension |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Applications： | The antibody binds to and neutralizes CAV-1 and is type specific. Tested and found negative for ectromelia virus (mousepox). |
| Storage Conditions： | liquid nitrogen vapor phase |
| Derivation： | Animals were immunized with purified CAV-1 (Mirandola strain). Spleen cells were fused with Sp2/0-Ag14 myeloma cells. |
| Genes Expressed： | immunoglobulin; monoclonal antibody; against canine adenovirus type 1 (CAV-1) |
| Cellular Products： | immunoglobulin; monoclonal antibody; against canine adenovirus type 1 (CAV-1) |
| Tumorigenic： | Yes |
| Effects： | Yes, in BALB/c mice |
| Comments： | Animals were immunized with purified CAV-1 (Mirandola strain). Spleen cells were fused with Sp2/0-Ag14 myeloma cells. The antibody binds to and neutralizes CAV-1 and is type specific. Tested and found negative for ectromelia virus (mousepox). |
| Complete Growth Medium： | Dulbeccos modified Eagles medium with 4.5 g/L glucose, 92.5%; horse serum, 5%; fetal bovine serum, 2.5%
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| Subculturing： | Cultures can be maintained by addition of fresh medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 2 x 105 viable cells/mL.xa0 Maintain cultures at a cell concentration between 1 x 105 and 1 x 106 cells/mL. Do not allow the cell concentration to exceed 1 x 106 cells/mL. Medium Renewal: Two to three times weekly |
| Cryopreservation： | Complete growth medium described above supplemented with 5% (v/v) DMSO.xa0 Cell culture tested DMSO is available as ATCC Catalog No. 4-X. |
| Culture Conditions： | Temperature: 37°C
Atmosphere: Air, 95%; Carbon dioxide (CO2), 5% |
| Isotype： | IgG1 |
| Name of Depositor： | CA Whetstone |
| Deposited As： | mouse (B cell); mouse (myeloma) |
| References： | Whetstone CA. Monoclonal antibodies to canine adenoviruses 1 and 2 that are type- specific by virus neutralization and immunofluorescence. Vet. Microbiol. 16: 1-8, 1988. PubMed: 3281371 Whetstone CA. Should the criteria for species distinction in adenoviruses be reconsidered? Evidence from canine adenoviruses 1 and 2. Intervirology 23: 116-120, 1985. PubMed: 3884549 Hay, R. J., Caputo, J. L., and Macy, M. L., Eds. (1992), ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC. Caputo, J. L., Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988. Fleming, D.O., Richardson, J. H., Tulis, J.J. and Vesley, D., (1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC. |