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| 产品名称： | 2G3 |
|---|---|
| 商品货号： | TS212935 |
| Organism： | Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma) |
| Cell Type： | hybridoma: B lymphocyte |
| Product Format： | frozen |
| Morphology： | lymphoblast |
| Culture Properties： | mixed: adherent and suspension |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Applications： | The cell line was established by Louis Gastinel and Pamela Bjorkman in 1992. Spleen cells were fused with HL-1 Friendly myeloma cells (a derivative of P3X63Ag8.653 that is deficient in both hypoxanthine phosphoribosyl transferase HPRT and adenine phosphoribosyl transferase APRT). FcRn is a heterodimer composed of a membrane bound heavy chain attached noncovalently to beta 2-microgloublin. This antibody is used in studies of the MHC class I heavy chain FcRn heterodimers and their interaction with IgG. |
| Storage Conditions： | liquid nitrogen vapor phase |
| Derivation： | The cell line was established by Louis Gastinel and Pamela Bjorkman in 1992. Animals were immunized with and twice boosted with soluble neonatal Fc receptor (FcRn) in an adjuvant. Three days before fusion one mouse was boosted with purified FcRn. Spleen cells were fused with HL-1 Friendly myeloma cells (a derivative of P3X63Ag8.653 that is deficient in both hypoxanthine phosphoribosyl transferase HPRT and adenine phosphoribosyl transferase APRT). |
| Genes Expressed： | immunoglobulin; monoclonal antibody; against FcRn heavy chain heterodimers |
| Cellular Products： | immunoglobulin; monoclonal antibody; against FcRn heavy chain heterodimers |
| Comments： | The cell line was established by Louis Gastinel and Pamela Bjorkman in 1992. Animals were immunized with and twice boosted with soluble neonatal Fc receptor (FcRn) in an adjuvant. Three days before fusion one mouse was boosted with purified FcRn. Spleen cells were fused with HL-1 Friendly myeloma cells (a derivative of P3X63Ag8.653 that is deficient in both hypoxanthine phosphoribosyl transferase HPRT and adenine phosphoribosyl transferase APRT). The antibody recognizes FcRn heavy chain heterodimers. FcRn is a heterodimer composed of a membrane bound heavy chain attached noncovalently to beta 2-microgloublin. It is structurally similar to class I major histocompatibility (MHC) molecules. The IG3 (CRL-2434) antibody recognizes two lipid linked forms of FcRn. These forms are truncated FcRn heavy chain paired with beta 2-microgloublin-DAF (FcRn beta-2 microglobulin DAF) and lipid-linked FcRn paired with unaltered beta 2-microgloublin (FcRn-DAF/beta 2-microgloublin). It also recognizes the FcRn-DAF construct. The 2G3 (CRL-2435) antibody fails to recognize the FcRn heterodimer form in which the heavy chain is attached to the lipid anchor (FcRn-DAF/beta 2-microgloublin) and it also fails to recognize the FcRn-DAF construct. Unlike 4C9 (CRL-2437), the monoclonal antibodies IG3 (CRL-2434)and 2G3 (CRL-2435) do not recognize rat beta 2-microglobulin alone or complexed with FcRn heavy chains. This antibody is used in studies of the MHC class I heavy chain FcRn heterodimers and their interaction with IgG. |
| Complete Growth Medium： | HL-1 medium supplemented with 4 mM L-glutamine, 1 mM sodium pyruvate and 1% fetal bovine serum. HL-1 medium can be obtained from from Lonza (catalog number 77201).
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| Subculturing： | Subcultures are prepared by scraping. Dislodge cells from the flask substrate with a cell scraper. Aspirate gently and dispense into new flasks. Seed flasks at 2xa0x 105 viable cells/mL. Subculture Ratio: 1:5 every 3 to 4 days. Medium Renewal: Add fresh medium every 2 to 3 days (depending on cell density) |
| Cryopreservation： | HL-1 Complete Serum-free medium described above supplemented with 20% (v/v) fetal bovine serum and 5% (v/v) DMSO. Cell culture tested DMSO is available as ATCC Catalog No. 4-X. |
| Culture Conditions： | Temperature: 37°C
Atmosphere: Air, 95%; Carbon dioxide (CO2), 5% |
| Isotype： | IgG1 |
| Name of Depositor： | PJ Bjorkman |
| Deposited As： | mouse (B cell); mouse (myeloma) |
| Year of Origin： | 1992 |
| References： | Raghavan M, et al. Investigation of the interaction between the class I MHC-related Fc receptor and its immunoglobulin G ligand. Immunity 1: 303-315, 1994. PubMed: 7889418 Hay, R. J., Caputo, J. L., and Macy, M. L., Eds. (1992), ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC. Caputo, J. L., Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988. Fleming, D.O., Richardson, J. H., Tulis, J.J. and Vesley, D., (1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC. |