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| 产品名称： | 2E6 |
|---|---|
| 商品货号： | TS212936 |
| Organism： | Cricetulus migratorius (B cell); Mus musculus (myeloma), hamster, Armenian (B cell); mouse (myeloma) |
| Cell Type： | hybridoma:lymphoblast B lymphocyte |
| Product Format： | frozen |
| Morphology： | lymphoblast |
| Culture Properties： | suspension |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Applications： | The antibody reacts precipitates a 150000/90000 dalton CD11/CD18 heterodimer (see also ATCC HB-225) from murine leukocytes. |
| Storage Conditions： | liquid nitrogen vapor temperature |
| Derivation： | Spleen cells were fused with Sp2/0 myeloma cells. |
| Genes Expressed： | immunoglobulin; monoclonal antibody; against mouse beta 2 leukocyte integrins (CD18) |
| Cellular Products： | immunoglobulin; monoclonal antibody; against mouse beta 2 leukocyte integrins (CD18) |
| Comments： | Animals were immunized with mouse dendritic cells. Spleen cells were fused with Sp2/0 myeloma cells. The antibody reacts precipitates a 150000/90000 dalton CD11/CD18 heterodimer (see also ATCC HB-225) from murine leukocytes. 2E6 blocks the binding function of LFA-1 (CD11a) and CR3/Mac-1 (CD11b). |
| Complete Growth Medium： | The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium:
|
| Subculturing： | Protocol: Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 1 X 10 exp5 viable cells/ml.
Maintain cell density between 1 X 10 exp5 and 1 X 10 exp6 viable cells/ml. Medium Renewal: Every 2 to 3 days |
| Cryopreservation： | Freeze medium: Complete growth medium, 95%; DMSO, 5% Storage temperature: liquid nitrogen vapor temperature |
| Culture Conditions： | Temperature: 37.0°C |
| Isotype： | IgG |
| Name of Depositor： | RM Steinman |
| Deposited As： | hamster, Armenian (B cell); mouse (myeloma) |
| References： | Metlay JP, et al. The distinct leukocyte integrins of mouse spleen dendritic cells as identified with new hamster monoclonal antibodies. J. Exp. Med. 171: 1753-1771, 1990. PubMed: 2185332 |