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微信小章| 产品名称： | 2D12 |
|---|---|
| 商品货号： | TS212946 |
| Organism： | Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma) |
| Cell Type： | hybridoma: B lymphocyte |
| Product Format： | frozen |
| Morphology： | lymphoblast |
| Culture Properties： | suspension |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Applications： | Tested and found negative for ectromelia virus (mousepox). |
| Storage Conditions： | liquid nitrogen vapor phase |
| Derivation： | Spleen cells were fused with P3X63Ag8.653 myeloma cells. |
| Antigen Expression： | H-2d |
| Genes Expressed： | immunoglobulin; monoclonal antibody; against yellow fever virus (vaccine strains and Asibi strain),H-2d |
| Cellular Products： | immunoglobulin; monoclonal antibody; against yellow fever virus (vaccine strains and Asibi strain) |
| Comments： | Mice were immunized with the 17D strain of yellow fever virus. Spleen cells were fused with P3X63Ag8.653 myeloma cells. The antibody does not cross-react with other flaviviruses. Antibody reactivity can be assayed by hemagglutination inhibition, complement fixation and immunofluorescence. Tested and found negative for ectromelia virus (mousepox). |
| Complete Growth Medium： | RPMI 1640 medium containing non-essential amino acids, 20 mM HEPES, 1 mM sodium pyruvate, 2 mM L-glutamine, and 0.02 mM 2-mercaptoethanol, 85%; fetal bovine serum, 15%
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| Subculturing： | Cultures can be maintained by addition of fresh medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 1 x 105 viable cells/mL.xa0 Maintain cultures at a cell concentration between 1 x 105 and 1 x 106 cells/mL. Do not allow the cell concentration to exceed 1 x 106 cells/mL.
Medium Renewal: Two to three times weekly
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| Cryopreservation： | Complete growth medium described above supplemented with 5% (v/v) DMSO.xa0 Cell culture tested DMSO is available as ATCC Catalog No. 4-X. |
| Culture Conditions： | Temperature: 37°C
Atmosphere: Air, 95%; Carbon dioxide (CO2), 5% |
| Isotype： | IgG2a; kappa light chain |
| Name of Depositor： | JJ Schlesinger |
| Deposited As： | mouse (B cell); mouse (myeloma) |
| References： | Schlesinger JJ, et al. Monoclonal antibodies distinguish between wild and vaccine strains of yellow fever virus by neutralization, hemagglutination inhibition and immune precipitation of the virus envelope protein. Virology 125: 8-17, 1983. PubMed: 6187129 Monath TP, et al. Yellow fever monoclonal antibodies: type-specific and cross-reactive determinants identified by immunofluorescence. Am. J. Trop. Med. Hyg. 33: 695-698, 1984. PubMed: 6206738 Hay, R. J., Caputo, J. L., and Macy, M. L., Eds. (1992), ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC. Caputo, J. L., Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988. Fleming, D.O., Richardson, J. H., Tulis, J.J. and Vesley, D., (1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC. |