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微信小章| 产品名称： | 293/CHE-Fc |
|---|---|
| 商品货号： | TS212949 |
| Organism： | Homo sapiens, human |
| Tissue： | kidney |
| Product Format： | frozen |
| Morphology： | epithelial |
| Culture Properties： | adherent |
| Biosafety Level： | 2 CELLS CONTAIN ADENOVIRUS
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease： | Influenza |
| Age： | fetus |
| Applications： | 293/CHE-Fc was established by cotransfecting human embryonic 293 cell (ATCC CRL-1573) with the plasmid pCHE-Fc and a plasmid encoding Genetic (G418) resistance. The cell line is stably transfected to secrete the chimeric protein (CHE-Fc), a recombinant soluble chirmeric protein useful as a probe to detect 9-O-Acetylated sialic acids. CHE-Fc is a bifunctional molecule that can be used either to cleave acetyl groups at the ninth position of sialic acids or as a binding probe for 9-O-sialic acids. |
| Storage Conditions： | liquid nitrogen vapor phase |
| Derivation： | 293/CHE-Fc was established by cotransfecting human embryonic 293 cell (ATCC CRL-1573) with the plasmid pCHE-Fc and a plasmid encoding Genetic (G418) resistance. |
| Genes Expressed： | soluble CHE-Fc |
| Cellular Products： | soluble CHE-Fc |
| Comments： | 293/CHE-Fc was established by cotransfecting human embryonic 293 cell (ATCC CRL-1573) with the plasmid pCHE-Fc and a plasmid encoding Genetic (G418) resistance. The cell line is stably transfected to secrete the chimeric protein (CHE-Fc), a recombinant soluble chirmeric protein useful as a probe to detect 9-O-Acetylated sialic acids. CHE-Fc is the fusion protein of hemagglutinin-esterase from Influenza C with human IgG1 Fc. CHE-Fc is a bifunctional molecule that can be used either to cleave acetyl groups at the ninth position of sialic acids or as a binding probe for 9-O-sialic acids. 293/CHE-Fc cells are G418 resistant. |
| Complete Growth Medium： | Dulbeccos modified Eagles medium with 4 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate and 4.5 g/L glucose and supplemented with 1 mg/ml G-418, 90%; heat inactivated fetal bovine serum, 10%.
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| Subculturing： | Volumes used in this protocol are for 75 cm2 flask; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes.
Subcultivation Ratio: 1:6 to 1:10 Note: For more information on enzymatic dissociation and subculturing of cell lines consult Chapter 10 in Culture of Animal Cells, a Manual of Basic Technique by R. Ian Freshney, 3rd edition, published by Alan R. Liss, N.Y., 1994. |
| Cryopreservation： | Complete growth medium described above supplemented with 5% (v/v) DMSO.xa0 Cell culture tested DMSO is available as ATCC Catalog No. 4-X. |
| Culture Conditions： | Temperature: 37°C
Atmosphere: Air, 95%; Carbon dioxide (CO2), 5% |
| Population Doubling Time： | 24 hrs |
| Name of Depositor： | AP Varki |
| Deposited As： | Homo sapiens |
| References： | Klein A, et al. 9-O-Acetylated sialic acids have widespread but selective expression: Analysis using a chimeric dual-function probe derived from influenza C hemaggIutinin-estrase. Proc. Natl. Acad. Sci. USA 91: 7782-7786, 1994. PubMed: 8052660 Hay, R. J., Caputo, J. L., and Macy, M. L., Eds. (1992), ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC. Caputo, J. L., Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988. Biosafety in Microbiological and Biomedical Laboratories, 5th ed. HHS. U.S. Department of Health and Human Services, Centers for Disease Control and Prevention. Washington DC: U.S. Government Printing Office; 2007. The entire text is available online. |