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微信小章| 产品名称： | 260-33C4 |
|---|---|
| 商品货号： | TS212963 |
| Organism： | Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma) |
| Tissue： | spleen |
| Cell Type： | hybridoma: B lymphocyte |
| Product Format： | frozen |
| Morphology： | lymphoblast |
| Culture Properties： | suspension |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Age： | adult |
| Strain： | 129GIX+ |
| Storage Conditions： | liquid nitrogen vapor phase |
| Derivation： | Spleen cells were fused with Sp2/0 myeloma cells. |
| Genes Expressed： | immunoglobulin; monoclonal antibody; against a RAF 1 synthetic oncogene peptide |
| Cellular Products： | immunoglobulin; monoclonal antibody; against a RAF 1 synthetic oncogene peptide |
| Comments： | Spleen cells were fused with Sp2/0 myeloma cells. |
| Complete Growth Medium： | The base medium for this cell line is ATCC-formulated Dulbeccos Modified Eagles Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
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| Subculturing： | Protocol: Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 1 to 2 X 10 exp5 viable cells/ml.
Maintain cell density between 1 X 10 exp5 and 1 X 10 exp6 viable cells/ml. Medium Renewal: Add fresh medium every 2 to 3 days (depending on cell density) |
| Cryopreservation： | Freeze medium: Complete growth medium 95%; DMSO, 5% Storage temperature: liquid nitrogen vapor phase |
| Culture Conditions： | Temperature: 37.0°C |
| Isotype： | IgM |
| Name of Depositor： | National Cancer Institute |
| Deposited As： | mouse (B cell); mouse (myeloma) |
| References： | Animals were immunized with a synthetic polypeptide with the sequence LVADCWKKVKEERPLF corresponding to peptides 584 to 599 of the RAF-1 oncogene (formerly v-raf residues 255 to 270). In ELISA testing, the antibody cross reacts with the homologous region of MET, sea, ros, src, SRC, yes, PKS, FMS, HIR, lck, kit. Weak activity was detected for FGR, c-mos, v-mos, MOS, erb-B, v-fms. In immunoblot testing, proteins of approximately 80 and 100 kd are detected in fms transformed 3T3 cells. The IgM and IgG2b reactivities of the antibody with the RAF-1 peptide (584 to 599) were confirmed by ELISA. IgM was strongly positive while IgG2b was negative. |