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微信小章| 产品名称： | 266-6 |
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| 商品货号： | TS212964 |
| Organism： | Mus musculus, mouse |
| Tissue： | pancreas |
| Product Format： | frozen |
| Morphology： | epithelial |
| Culture Properties： | adherent |
| Biosafety Level： | 2 xa0Cells contain Papovavirus
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease： | pancreatic acinar cell tumor |
| Age： | adult |
| Applications： | The 266-6 cell line is useful for transfection studies. |
| Storage Conditions： | liquid nitrogen vapor phase |
| Derivation： | 266-6 is an acinar pancreatic cell line derived in 1985 by Robert E. Hammer from a young adult mouse.
The tumor was induced with an elastase I/SV-40 T antigen fusion gene.
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| Receptor Expression： | acetylcholine, muscarinic |
| Comments： | These cells retain a partially differentiated phenotype, and express detectable levels of a number of digestive enzyme mRNAs.
The cells respond to carbachol and cholecystokinin but not to substance P, secretin, or vasoactive intestinal peptide (VIP).
They bear an elastase I/neomycin transgene.
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| Complete Growth Medium： | The base medium for this cell line is ATCC-formulated Dulbeccos Modified Eagles Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
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| Subculturing： | Volumes used in this protocol are for 75 cm2 flask; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes. Cells must be seeded on 0.1% gelatin-coated culturexa0 vessels. To make the coating, dissolve 1.0 g of Gelatin Type A (Sigma G2500) in 1L of distilled water, sterilize by autoclaving. Add 0.4 to 0.5 mL of gelatin solution per cm2 of the culture vessel and refrigerate for 10 to 15 minutes. Prior to use remove the gelatin solution and rinse gently with culture medium, adding at least 0.1 to 0.2 ml per cm2. Discard the culture medium used for rinsing and use vessels immediately. If required, coated vessels can be prepared in advance and stored at 2 to 8˚C no more than 5 days.
Subculture Ratio: 1:3 to 1:4 Medium Renewal: 2 to 3 times a week.
Note: For more information on enzymatic dissociation and subculturing of cell lines consult Chapter 10 in Culture of Animal Cells, a manual of Basic Technique by R. Ian Freshney, 3rd edition, published by Alan R. Liss, N.Y., 1994. |
| Cryopreservation： | Freeze medium: Complete growth medium, 95%; DMSO, 5% Storage temperature: liquid nitrogen vapor phase |
| Name of Depositor： | GH Swift |
| Deposited As： | Mus musculus |
| Year of Origin： | 1985 |
| References： | Kruse F, et al. The cell-specific elastase I enhancer comprises two domains published erratum appears in Mol. Cell. Biol. 8: 1862, 1988. Mol. Cell. Biol. 8: 893-902, 1988. PubMed: 3352608 Swift GH, et al. Differential requirements for cell-specific elastase I enhancer domains in transfected cells and transgenic mice. Genes Dev. 3: 687-696, 1989. PubMed: 2744460 Ornitz DM, et al. Elastase I promoter directs expression of human growth hormone and SV40 T antigen genes to pancreatic acinar cells in transgenic mice. Cold Spring Harbor Symp. Quant. Biol. 50: 399-409, 1985. PubMed: 3006998 Rose SD, et al. A single element of the elastase I enhancer is sufficient to direct transcription selectively to the pancreas and gut. Mol. Cell. Biol. 14: 2048-2057, 1994. PubMed: 8114736 Swift GH, et al. An element of the elastase I enhancer is an overlapping bipartite binding site activated by a heteromeric factor. J. Biol. Chem. 269: 12809-12815, 1994. PubMed: 8175694 |