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| 产品名称： | 225 |
|---|---|
| 商品货号： | TS212968 |
| Organism： | Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma) |
| Cell Type： | hybridoma: B lymphocyte |
| Product Format： | frozen |
| Morphology： | lymphoblast |
| Culture Properties： | suspension |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Applications： | The antibody reacts with EGF receptors, blocks the binding of EGF to the receptor and inhibits the growth of A-431 and T222 tumors in nude mice (although not as strongly as 528 (see ATCC HB-8509). The antibody binds to a single class of receptor sites on A-431, HeLa and human fibroblasts, and it blocks EGF stimulated phosphorylation of membrane proteins. |
| Storage Conditions： | liquid nitrogen vapor phase |
| Disclosure： | This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC. |
| Derivation： | Animals were immunized with partially purified EGF receptors from A-431 cells. Spleen cells were fused with NS-1-503 myeloma cells. |
| Cellular Products： | immunoglobulin; monoclonal antibody; against epidermal growth factor (EGF) receptor |
| Tumorigenic： | Yes |
| Effects： | Yes, in pristane primed BALB/cJ mice |
| Comments： | Animals were immunized with partially purified EGF receptors from A-431 cells. Spleen cells were fused with NS-1-503 myeloma cells. The antibody reacts with EGF receptors, blocks the binding of EGF to the receptor and inhibits the growth of A-431 and T222 tumors in nude mice (although not as strongly as 528 (see ATCC HB-8509). The antibody binds to a single class of receptor sites on A-431, HeLa and human fibroblasts, and it blocks EGF stimulated phosphorylation of membrane proteins. |
| Complete Growth Medium： | RPMI 1640 medium with 2 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate, 4.5 g/L glucose, 10 mM HEPES, and 1.0 mM sodium pyruvate, 85%; horse serum, 15%
|
| Subculturing： | Protocol: Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml. Medium Renewal: Add fresh medium every 2 to 3 days (depending on cell density) |
| Cryopreservation： | Freeze medium: Complete growth medium, 95%; DMSO, 5% Storage temperature: liquid nitrogen vapor phase |
| Culture Conditions： | Temperature: 37.0°C |
| Isotype： | IgG1 |
| Antigenic Determinant： | EGF receptor |
| Name of Depositor： | Regents of the University of California |
| Deposited As： | mouse (B cell); mouse (myeloma) |
| U.S. Patent Number： | |
| References： | Mendelsohn J, et al. Hybrid cell lines that produce monoclonal antibodies to epidermal growth factor receptor. US Patent 4,943,533 dated Jul 24 1990 Sato JD, et al. Biological effects in vitro of monoclonal antibodies to human epidermal growth factor receptors. Mol. Biol. Med. 1: 511-529, 1983. PubMed: 6094961 |