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微信小章| 产品名称： | 20C2 4H12:20C2.G6.C9 |
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| 商品货号： | TS212969 |
| Organism： | Rattus norvegicus (B cell); Mus musculus (myeloma), rat (B cell); mouse (myeloma) |
| Cell Type： | hybridoma: B lymphocyte |
| Product Format： | frozen |
| Morphology： | lymphoblast |
| Culture Properties： | suspension |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Applications： | The hybridoma cell line 20C2 4H12:20C2.G6.C9} was established by Richard Chizzonite in 1991 and subcloned three times by limiting dilution. The hybridoma produces an antibody (IgG1) that binds the p75 heterodimer of human IL-12. The antibody may be used in neutralizing and receptor binding assays; it will immunoprecipitate IL-12. The antibody can be used as a capture antibody in sandwich EIA to detect the p75 form of human IL-12. |
| Storage Conditions： | liquid nitrogen vapor phase |
| Derivation： | The hybridoma cell line 20C2 4H12:20C2.G6.C9} was established by Richard Chizzonite in 1991 and subcloned three times by limiting dilution. The 20C2 cell line was formed by the fusion of NSO mouse myeloma cells with splenocytes from a Lewis rat inoculated with partially purified human IL-12 p75. |
| Genes Expressed： | immunoglobulin; monoclonal antibody; against human interleukin 12 (IL-12) p75 |
| Cellular Products： | immunoglobulin; monoclonal antibody; against human interleukin 12 (IL-12) p75 |
| Comments： | The hybridoma cell line 20C2 4H12:20C2.G6.C9} was established by Richard Chizzonite in 1991 and subcloned three times by limiting dilution. The hybridoma produces an antibody (IgG1) that binds the p75 heterodimer of human IL-12. The antibody may be used in neutralizing and receptor binding assays; it will immunoprecipitate IL-12. The antibody can be used as a capture antibody in sandwich EIA to detect the p75 form of human IL-12. The 20C2 antibody does not bind the p40 or p35 subunits of IL-12. The 20C2 cell line was formed by the fusion of NSO mouse myeloma cells with splenocytes from a Lewis rat inoculated with partially purified human IL-12 p75. |
| Complete Growth Medium： | Iscoves Modified Dulbeccos Medium with 4 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate supplemented with 0.1 mM 2-mercaptoethanol, 90%; fetal bovine serum, 10%.
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| Subculturing： | Cultures can be maintained by addition of fresh Medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 1 to 2 x 105 viable cells/mL.xa0 Maintain cultures at a cell concentration between 1 x 105 and 1 x 106 cells/mL. Maintain cell density between 1 x 105 and 1 x 106 viable cells/mL. Medium Renewal: Add fresh medium every 2 to 3 days (as cell density increases) |
| Cryopreservation： | Complete growth medium described above supplemented with 5% (v/v) DMSO.xa0 Cell culture tested DMSO is available as ATCC Catalog No. 4-X. |
| Culture Conditions： | Temperature: 37°C
Atmosphere: Air, 95%; Carbon dioxide (CO2), 5% |
| Isotype： | IgG1 |
| Name of Depositor： | DH Presky |
| Deposited As： | rat (B cell); mouse (myeloma) |
| Year of Origin： | 1991 |
| References： | Chua AO, et al. Expression cloning of a human IL-12 receptor component--a new member of the cytokine receptor superfamily with strong homology to gp130. J. Immunol. 153: 128-136, 1994. PubMed: 7911493 Chizzonite R, et al. IL-12: monoclonal antibodies specific for the 40-kDa subunit block receptor binding and biologic activity on activated human lymphoblasts. J. Immunol. 147: 1548-1556, 1991. PubMed: 1715362 Chizzonite R, et al. IL-12 receptor. I. Characterization of the receptor on phytohemagglutinin-activated human lymphoblasts. J. Immunol. 148: 3117-3124, 1992. PubMed: 1578138 Desai BB, et al. IL-12 receptor. II. Distribution and regulation of receptor expression. J. Immunol. 148: 3125-3132, 1992. PubMed: 1578139 Gately MK, et alMeasurement of Human and Mouse Interleukin-12In: Gately MK, et alCurrent Protocols in ImmunologyNew York, N.Y.John Wiley and Sons1995. Hay, R. J., Caputo, J. L., and Macy, M. L., Eds. (1992), ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC. Caputo, J. L., Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988. Fleming, D.O., Richardson, J. H., Tulis, J.J. and Vesley, D., (1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC. Biosafety in Microbiological and Biomedical Laboratories, 5th ed. HHS. U.S. Department of Health and Human Services, Centers for Disease Control and Prevention. Washington DC: U.S. Government Printing Office; 2007. The entire text is available online. |