微信小陈
微信小章| 产品名称： | 2.4G2 |
|---|---|
| 商品货号： | TS212981 |
| Organism： | Rattus norvegicus (B cell); Mus musculus (myeloma), rat (B cell); mouse (myeloma) |
| Cell Type： | Hybridoma: B lymphocyte |
| Product Format： | frozen |
| Morphology： | Lymphoblast-like |
| Culture Properties： | Suspension |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Applications： | The antibody can be used to block non-specific binding to Fc gamma bearing cells. |
| Storage Conditions： | liquid nitrogen vapor phase |
| Derivation： | Animals were immunized with the J774 mouse macrophage cell line.xa0 Spleen cells were fused with P3U1 myeloma cells. |
| Genes Expressed： | immunoglobulin; monoclonal antibody; against the Fc gamma receptor (FcRII, CD32) |
| Cellular Products： | immunoglobulin; monoclonal antibody; against the Fc gamma receptor (FcRII, CD32) |
| Tumorigenic： | Yes |
| Effects： | Yes, in irradiated pristane primed BALB/c mice. |
| Comments： | The antibody reacts with and immunoprecipitates the 50000 dalton to 70000 dalton Fc gamma receptor on macrophages and Fc gamma bearing lymphoid cells. Tested and found negative for ectromelia virus (mousepox). |
| Complete Growth Medium： | Dulbeccos modified Eagles medium with 4 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate and 4.5 g/L glucose, 90%; horse serum, 5%; fetal bovine serum, 5%
|
| Subculturing： | Cultures can be maintained by addition of fresh medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 1 x 105xa0viable cells/mL. Maintain cultures at a cell concentration between 1 x 105 and 1 x 106xa0cells/mL. Do not allow the cell concentration to exceed 1 x 106xa0cells/mL. Medium Renewal:xa0Add fresh medium every 2 to 3 days (depending on cell density) |
| Cryopreservation： | culture medium 95%; DMSO, 5% |
| Culture Conditions： | Temperature: 37°C
Atmosphere: Air, 95%; Carbon dioxide (CO2), 5% |
| Isotype： | IgG2b |
| Name of Depositor： | JC Unkeless |
| Deposited As： | rat (B cell); mouse (myeloma) |
| References： | Unkeless JC. Characterization of a monoclonal antibody directed against mouse macrophage and lymphocyte Fc receptors. J. Exp. Med. 150: 580-596, 1979. PubMed: 90108 Mellman IS, Unkeless JC. Purification of a functional mouse Fc receptor through the use of a monoclonal antibody. J. Exp. Med. 152: 1048-1069, 1980. PubMed: 6158545 Nussenzweig MC, et al. Studies of the cell surface of mouse dendritic cells and other leukocytes. J. Exp. Med. 154: 168-187, 1981. PubMed: 7252426 Yoshikai Y, et al. Clonal expansion of superantigen-reactive T cells is resistant to FK506 in mice with AIDS. J. Virol. 71: 746-749, 1997. PubMed: 8985410 Wilson ME, et al. Local suppression of IFN-gamma in hepatic granulomas correlates with tissue-specific replication of Leishmania chagasi. J. Immunol. 156: 2231-2239, 1996. PubMed: 8690913 Hay, R. J., Caputo, J. L., and Macy, M. L., Eds. (1992), ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC. Caputo, J. L., Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988. Fleming, D.O., Richardson, J. H., Tulis, J.J. and Vesley, D., (1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC. |