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| 产品名称： | 200-3-G6-4 (20.4) |
|---|---|
| 商品货号： | TS212983 |
| Organism： | Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma) |
| Cell Type： | hybridoma: B lymphocyte |
| Product Format： | frozen |
| Morphology： | lymphoblast |
| Culture Properties： | suspension |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Applications： | The antibody binds to the 75000 dalton NGF receptor of primates. |
| Storage Conditions： | liquid nitrogen vapor phase |
| Disclosure： | This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC. |
| Derivation： | Spleen cells were fused with P3X63Ag8 myeloma cells. |
| Genes Expressed： | immunoglobulin; monoclonal antibody; against nerve growth factor (NGF) receptor of primates |
| Cellular Products： | immunoglobulin; monoclonal antibody; against nerve growth factor (NGF) receptor of primates |
| Tumorigenic： | Yes |
| Effects： | Yes, forms ascites in BALB/c mice |
| Comments： | Animals were immunized with human melanoma cell lines. Spleen cells were fused with P3X63Ag8 myeloma cells. The antibody binds to the 75000 dalton NGF receptor of primates. |
| Complete Growth Medium： | Iscoves modified Dulbeccos medium with 2 mM L-glutamine, 90%; fetal bovine serum, 10%
|
| Subculturing： | Protocol: Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 1 X 10(5) cells/ml and maintain between 1 X 10(5) and 1 X 10(6) cells/ml. Medium Renewal: every 2 to 3 days |
| Cryopreservation： | Freeze medium: Culture medium, 95%; DMSO, 5% Storage temperature: liquid nitrogen vapor phase |
| Culture Conditions： | Temperature: 37.0°C |
| Isotype： | IgG1 |
| Name of Depositor： | Wistar Institute |
| Deposited As： | mouse (B cell); mouse (myeloma) |
| U.S. Patent Number： | |
| References： | Koprowski H, et al. Diagnostic method for detection of neural crest disease. US Patent 4,786,593 dated Nov 22 1988 Johnson EM Jr.. Tumor diagnostic method. US Patent 4,855,241 dated Aug 8 1989 Levi AD, et al. The functional characteristics of Schwann cells cultured from human peripheral nerve after transplantation into a gap within the rat sciatic nerve. J. Neurosci. 14: 1309-1319, 1994. PubMed: 8120626 |