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微信小章| 产品名称： | 1G10 |
|---|---|
| 商品货号： | TS212990 |
| Organism： | Rattus norvegicus (B cell); Mus musculus (myeloma), rat (B cell); mouse (myeloma) |
| Tissue： | lymph node, popliteal |
| Cell Type： | hybridoma: B lymphocyte |
| Product Format： | frozen |
| Morphology： | lymphoblast |
| Culture Properties： | suspension |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease： | Lymphoma |
| Age： | adult |
| Strain： | Lewis |
| Applications： | The antibody will neutralize the B7.1 antigen, but does not react with the B7.2 antigen. |
| Derivation： | Popliteal lymph node cells were fused with Sp2/0-Ag14 myeloma cells. |
| Genes Expressed： | immunoglobulin; monoclonal antibody; against mouse B7.1 |
| Cellular Products： | immunoglobulin; monoclonal antibody; against mouse B7.1 |
| Comments： | Animals were immunized in the hind footpad with irradiated 5C2 mouse B cell lymphoma cells that had been cultured in the presence of dibutyryl-cAMP. Popliteal lymph node cells were fused with Sp2/0-Ag14 myeloma cells. The antibody will neutralize the B7.1 antigen, but does not react with the B7.2 antigen. |
| Complete Growth Medium： | The base medium for this cell line is ATCC-formulated Iscoves Modified Dulbeccos Medium, Catalog No. 30-2005. To make the complete growth medium, add the following components to the base medium:
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| Subculturing： | Medium Renewal: Every 2 to 3 days Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 0.5 X 10 exp5 to 1 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml. |
| Cryopreservation： | Culture medium, 95%; DMSO, 5% |
| Isotype： | IgG2a |
| Name of Depositor： | SE Buchholz |
| Deposited As： | rat (B cell); mouse (myeloma) |
| References： | Powers GD, et al. Expression and functional analysis of murine B7 delineated by a novel monoclonal antibody. Cell. Immunol. 153: 298-311, 1994. PubMed: 7509723 Nabavi N, et al. Signalling through the MHC class II cytoplasmic domain is required for antigen presentation and induces B7 expression. Nature 360: 266-268, 1992. PubMed: 1279442 Chen C, et al. Monoclonal antibody 2D10 recognizes a novel T cell costimulatory molecule on activated murine B lymphocytes. J. Immunol. 152: 2105-2114, 1994. PubMed: 7510738 |