微信小陈
微信小章| 产品名称： | 1D3 |
|---|---|
| 商品货号： | TS212994 |
| Organism： | Rattus norvegicus (B cell); Mus musculus (myeloma), rat (B cell); mouse (myeloma) |
| Cell Type： | hybridoma: B lymphocyte |
| Product Format： | frozen |
| Morphology： | lymphoblast |
| Culture Properties： | suspension |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Applications： | It is useful flow cytometric analysis of total mouse B cells and for studies of signal transduction involving CD19. |
| Storage Conditions： | liquid nitrogen vapor phase |
| Derivation： | The line was produced by fusing NS-1 myeloma cells with spleen cells from Lewis rats that had been immunized with transfected K562 cells expressing the extracellular domain of murine CD19. |
| Genes Expressed： | immunoglobulin; monoclonal antibody; against mouse CD19 |
| Cellular Products： | immunoglobulin; monoclonal antibody; against mouse CD19 |
| Comments： | The hybridoma cell line 1D3 secretes a rat monoclonal antibody that reacts with the mouse pan-B cell marker CD19. The antibody binds to mouse CD19 with high affinity, but does not react with human CD19. |
| Complete Growth Medium： | RPMI 1640 medium with 2 mM L-glutamine adjusted to contain
1.5 g/L sodium bicarbonate, 4.5 g/L glucose, 10 mM HEPES, and 1.0 mM sodium
pyruvate supplemented with 0.05 mM 2-mercaptoethanol, 90%; fetal bovine
serum, 10%
|
| Subculturing： | Cultures can be maintained by addition or replacement of fresh medium. Alternately, cultures can be established by centrifugation with subsequent resuspension in fresh culture medium. Establish new cultures at 2 x 105xa0viable cells/mL and maintain at between 1 x 105 and 1 x 106 viable cells/mL. Medium Renewal: Every 2 to 3 days |
| Cryopreservation： | Culture medium, 95%; DMSO, 5%.xa0Cell culture tested DMSO is available as ATCC Catalog No. 4-X. |
| Culture Conditions： | Temperature: 37°C |
| Isotype： | IgG2a; kappa light chain |
| Name of Depositor： | DT Fearon, I Krop, MS Schlissel, AR de Fougerolles |
| References： | Krop I, et al. Self-renewal of B-1 lymphocytes is dependent on CD19. Eur. J. Immunol. 26: 238-242, 1996. PubMed: 8566073 Hay, R. J., Caputo, J. L., and Macy, M. L., Eds. (1992), ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC. Caputo, J. L., Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988. Fleming, D.O., Richardson, J. H., Tulis, J.J. and Vesley, D., (1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC. |