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微信小章| 产品名称： | 1B2A3 |
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| 商品货号： | TS212998 |
| Organism： | Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma) |
| Cell Type： | hybridoma: B lymphocyte |
| Product Format： | frozen |
| Morphology： | lymphoblast |
| Culture Properties： | suspension |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Applications： | The antibody differentiates toxin producing strains of P. multocida from other isolates, and can be used to identify toxigenic strains of P. multocida. The 1B2A3 hybridoma was established by Kevin W. Ruby and Bill Knudtson in 1990. |
| Storage Conditions： | liquid nitrogen vapor phase |
| Derivation： | Animals were immunized with a toxoid preparation purified from the NOR-1 strain of P. multocida (the toxoid preparation was a gift from J.C. Frantz). Spleen cells were fused with Sp2/0-Ag14 myeloma cells. The 1B2A3 hybridoma was established by Kevin W. Ruby and Bill Knudtson in 1990. |
| Genes Expressed： | immunoglobulin; monoclonal antibody; against Pasturella multocida type D dermonecrotic toxin |
| Cellular Products： | immunoglobulin; monoclonal antibody; against Pasturella multocida type D dermonecrotic toxin |
| Tumorigenic： | Yes |
| Effects： | Yes, in BALB/c mice |
| Comments： | Animals were immunized with a toxoid preparation purified from the NOR-1 strain of P. multocida (the toxoid preparation was a gift from J.C. Frantz). Spleen cells were fused with Sp2/0-Ag14 myeloma cells. The antibody differentiates toxin producing strains of P. multocida from other isolates, and can be used to identify toxigenic strains of P. multocida. The 1B2A3 hybridoma was established by Kevin W. Ruby and Bill Knudtson in 1990. |
| Complete Growth Medium： | Dulbeccos modified Eagles medium with 4.5 g/L glucose, 92.5%; horse serum, 5%; fetal bovine serum, 2.5%
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| Subculturing： | Cultures can be maintained by addition of fresh medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 1 x 105 viable cells/mL. xa0Maintain cultures at a cell concentration between 2 x 105 and 1 x 106 cells/mL. Medium Renewal: Two to three times weekly |
| Cryopreservation： | Complete growth medium described above supplemented with 5% (v/v) DMSO.xa0 Cell culture tested DMSO is available as ATCC Catalog No. 4-X. |
| Culture Conditions： | Temperature: 37°C
Atmosphere: Air, 95%; Carbon dioxide (CO2), 5% |
| Isotype： | IgG1 |
| Name of Depositor： | LA Elsken Originators: KW Ruby,W Knudtson |
| Deposited As： | mouse (B cell); mouse (myeloma) |
| Year of Origin： | 1990 |
| References： | Martineau-Doize B, et al. Effects of purified Pasteurella multocida dermonecrotoxin on cartilage and bone of the nasal ventral conchae of the piglet. Anat. Rec. 228: 237-246, 1990. PubMed: 2260779 Magyar T, Rimler RB. Detection and enumeration of toxin-producing Pasteurella multocida with a colony-blot assay. J. Clin. Microbiol. 29: 1328-1332, 1991. PubMed: 1885729 Hay, R. J., Caputo, J. L., and Macy, M. L., Eds. (1992), ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC. Caputo, J. L., Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988. Fleming, D.O., Richardson, J. H., Tulis, J.J. and Vesley, D., (1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC. |