微信小陈
微信小章
产品简介
购买须知
| 产品名称： | 182-PF SK |
|---|---|
| 商品货号： | TS213008 |
| Organism： | Homo sapiens, human |
| Tissue： | skin |
| Product Format： | frozen |
| Morphology： | fibroblast |
| Culture Properties： | adherent |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease： | hereditary adenomatosis |
| Age： | 20 years |
| Gender： | male |
| Ethnicity： | Caucasian |
| Clinical Data： | The tissue donor suffered from hereditary adenomatosis. male Caucasian 20 years |
| Comments： | The tissue donor suffered from hereditary adenomatosis. The line is capable of 10 population doublings after thawing. |
| Complete Growth Medium： | The base medium for this cell line is ATCC-formulated Dulbeccos Modified Eagles Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
|
| Subculturing： | Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:3 is recommended Medium Renewal: Twice per week Remove medium, add fresh 0.25% trypsin solution for 1 to 3 minutes, remove trypsin and let the culture sit at room temperature for 5 to 10 minutes. Add fresh medium, aspirate and dispense into new flasks. Subculture every 10 days. |
| Name of Depositor： | L Kopelovich |
| Deposited As： | Homo sapiens |
| References： | Rhim JS, et al. Chemical transformation of cultured skin fibroblasts from humans genetically predisposed to cancer. Cancer Detect. Prev. 4: 239-247, 1981. PubMed: 7349781 Prog. Cancer Res. Ther. 13: 97-108, 1980. |