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| 产品名称： | 171-11B9 |
|---|---|
| 商品货号： | TS213009 |
| Organism： | Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma) |
| Cell Type： | hybridoma: B lymphocyte |
| Product Format： | frozen |
| Morphology： | lymphoblast |
| Culture Properties： | suspension |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Applications： | Animals were immunized with a synthetic peptide with the sequence IMVKCWMIDADSRPKF corresponding to peptides 946 to 961 of the HER 1 oncogene (formerly 366 to 381 of the v-erb-B oncogene). In addition, minor activities with proteins of 50-60 kd are detected in these cells as well as a number of additional cell lines and tissues. The IgG1 reactivity of the antibody with the corresponding peptide was confirmed by ELISA. IgG1 was strongly positive while IgG2b was only slightly positive. |
| Derivation： | Spleen cells were fused with Sp2/0 myeloma cells. |
| Genes Expressed： | immunoglobulin; monoclonal antibody; against a synthetic HER 1 oncogene peptide (formerly v-erb B) |
| Cellular Products： | immunoglobulin; monoclonal antibody; against a synthetic HER 1 oncogene peptide (formerly v-erb B) |
| Comments： | Animals were immunized with a synthetic peptide with the sequence IMVKCWMIDADSRPKF corresponding to peptides 946 to 961 of the HER 1 oncogene (formerly 366 to 381 of the v-erb-B oncogene). Spleen cells were fused with Sp2/0 myeloma cells. In immunoblot tests, the antibody detects proteins of 170 kd and 150 kd in A431 cells. In addition, minor activities with proteins of 50-60 kd are detected in these cells as well as a number of additional cell lines and tissues. In ELISA tests, the antibody does not detect the homologous region of fes, mos, src, yes, mil, raf, rel, fms, abl, ros, or human insulin receptor. The fgr peptide conjugated to keyhole limpet hemocyanin, however, is recognized by the monoclonal antibody. The IgG1 reactivity of the antibody with the corresponding peptide was confirmed by ELISA. IgG1 was strongly positive while IgG2b was only slightly positive. |
| Complete Growth Medium： | The base medium for this cell line is ATCC-formulated Dulbeccos Modified Eagles Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
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| Subculturing： | Medium Renewal: Add fresh medium every 2 to 3 days (depending on cell density) Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 1 to 2 X 10 exp5 viable cells/ml. Maintain cell density between 1 X 10 exp5 and 1 X 10 exp6 viable cells/ml. |
| Cryopreservation： | Culture medium with 45% fetal bovine serum, 90%; DMSO, 10% |
| Culture Conditions： | Temperature: 37.0°C |
| Isotype： | IgG1 and IgG2b |
| Name of Depositor： | National Cancer Institute |
| Deposited As： | mouse (B cell); mouse (myeloma) |